ArticleIranian biomedical journal2020
A Real-Time RT-PCR Assay for Genotyping of Rotavirus
Article in Iranian biomedical journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Epidemiology and genetic variation of acute viral gastroenteritis in children under five years in the Middle East (2020-2025): a systematic review and meta-analysis.BMC infectious diseases · 2026Pooled it
- The efficacy of lactobacillus reuteri in conjunction with racecadotril in managing pediatric rotavirus enteritis and its impact on intestinal mucosa and immune function.African health sciences · 2025Trial
- Dual role of Japanese encephalitis virus fusion loop peptide antibodies in Zika virus infection.PLoS neglected tropical diseases · 2026Article
- Development of RT-dPCR method and reference material for rotavirus G3P8 and G9P8.Analytical and bioanalytical chemistry · 2025Article
- A Nomogram Based on Immune Inflammation Indicators for Rotaviral Diarrhea in Children Under 5 years Old: A Single-Center Retrospective Cohort Study.Journal of inflammation research · 2025Article
- Possible viral agents to consider in the differential diagnosis of blepharoconjunctivitis.World journal of virology · 2024Article
- Efficient SARS-CoV-2 Surveillance during the Pandemic-Endemic Transition Using PCR-Based Genotyping Assays.Microbiology spectrum · 2023Article
- Article
- Development of a rapid homogeneous immunoassay for detection of rotavirus in stool samples.Frontiers in public health · 2022Article
- Detection and characterization of rotavirus G and P types from children with acute gastroenteritis in Qom, central Iran.Gastroenterology and hepatology from bed to bench · 2020Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Human rotavirus (HRV) is the causative agent of severe gastroenteritis in children and responsible for two million hospitalizations and more than a half-million deaths annually. Sequence characteristics of the gene segments encoding the VP7 and VP4 proteins are used for the genotype classification of rotavirus. A wide variety of molecular methods are available, mainly based on reverse transcription PCR for rapid, specific and sensitive genotyping of rotaviruses. This study describes an alternative real-time PCR assay for genotyping of rotavirus. Methods: The samples of stools studied in this research have been collected from patients referred to Children's Medical Centers, Tehran, Iran. Rotavirus detection and genotyping were performed using the RT-PCR and semi-nested RT-PCR, respectively. Samples were then genotyped with a new real-time PCR. Results: The real-time PCR was able to genotype all positive samples with a mean Ct of 28.2. Besides, a concordance rate of 100% was detected between real-time PCR and semi-nested RT-PCR. Conclusion: In this study, the genotyping of rotavirus with real-time PCR showed that this method can provide several favorable features, including high sensitivity and specificity, and a wide dynamic range for rotavirus genotyping.
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