Evidence map›Paper›PMID 32660680›Full record

ArticleParasitology2020

Mimotope-based antigens as potential vaccine candidates in experimental murine cysticercosis.

Marianna Nascimento Manhani, Cristiane Queixa Tilelli, Vanessa da Silva Ribeiro, Luiz Ricardo Goulart, Julia Maria Costa-Cruz

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasitology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Marianna Nascimento ManhaniLaboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, Minas Gerais, 38400-902, Brazil.
Cristiane Queixa TilelliLaboratório de Fisiologia, Universidade Federal de São João del-Rei, R. Sebastião Gonçalves Coelho 400, Divinópolis, Minas Gerais, 35501-296, Brazil.
Vanessa da Silva RibeiroLaboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, Minas Gerais, 38400-902, Brazil.
Luiz Ricardo GoulartLaboratório de Nanobiotecnologia, Instituto de Biotecnologia, Universidade Federal de Uberlândia, Av. Amazonas s/n, Bl. 2E, Uberlândia, Minas Gerais, 38400-902, Brazil.
Julia Maria Costa-CruzLaboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, Minas Gerais, 38400-902, Brazil.ORCID 0000-0001-5751-8511

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human cysticercosis is a public health problem caused by Taenia solium metacestodes; thus, eradication of T. solium transmission by vaccination is an urgent requirement. The Cc48 mimotope from T. solium cysticerci was tested expressed in phage particles (mCc48) and chemically synthesized (sCc48) as a vaccine candidate in experimental murine cysticercosis. For this, BALB/c mice were immunized with mCc48 (G1; n = 40), sCc48 (G2; n = 40) and phosphate-buffered saline (PBS) (G3; n = 40, positive control) and challenged with Taenia crassiceps metacestodes. Another PBS group without parasite challenge was used as a negative control (G4; n = 40). Mice were sacrificed 15, 30, 45 and 60 days post-infection for cysticerci and serum collection. Immunization efficacy was determined by cysticerci counting. Serum samples were tested by ELISA to verify antibody (IgM, IgG, IgA and IgE) and cytokine (IFNγ and IL-4) levels. The sCc48 achieved the highest rates of protection and efficacy (90 and 98%, respectively). The group immunized with mCc48 presented the highest reactivity for IgM, IgG and IgE. All groups presented IL-4, but IFNγ was quite variable among groups. The protection induced by sCc48 synthetic peptide supports further studies of this mimotope as a potential vaccine candidate against cysticercosis.

Indexed as

VaccinesAnimalsAntibodies, HelminthAntigens, HelminthCysticercosisCysticercusCytokinesHumansImmunityImmunizationMiceMice, Inbred BALB CTaeniaAntibodies, HelminthAntigens, HelminthCytokinesVaccinesCysticercosispeptide Cc48Taenia crassicepsvaccine

Identifiers

PMID32660680
PMCPMC10317767

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.