ArticleParasites & vectors2020
Echinococcus granulosus (sensu stricto) (G1, G3) and E. ortleppi (G5) in Pakistan: phylogeny, genetic diversity and population structural analysis based on mitochondrial DNA.
Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 2 of them syntheses that pooled it.
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Who cites it
24 citing papers in PubMed, 2 syntheses or guidelines pooled it, 42 citations in OpenAlex.
- Echinococcus granulosus sensu lato Genotypes in Different Hosts Worldwide: A Systematic Review.Acta parasitologica · 2022Pooled it
- A review of foodborneFrontiers in veterinary science · 2022Pooled it
- Seroprevalence and Risk Factors of Cystic Echinococcosis in Cattle and Buffaloes: Insights From an In-House ELISA.Veterinary medicine and science · 2026Article
- Three decades of research on molecular genetic characteristics of Echinococcus spp.: a bibliometric study.Folia parasitologica · 2026Review
- Five-Year Slaughterhouse-Based Surveillance ofPathogens (Basel, Switzerland) · 2025Article
- Genetic diversity study and phylogenetic analysis of Echinococcus granulosus sensu lato infecting buffaloes in India using NAD1, COX1 and ITS1 genes.Molecular biology reports · 2025Article
- Investigating the Epidemiology and Socioecological Dynamics of Hydatid-like Cysts Within a Specific Endemic District.Animals : an open access journal from MDPI · 2025Article
- Article
- Epidemiological and genotypic assessment of cystic echinococcosis in ruminant populations of Northern Punjab, Pakistan: a neglected zoonotic disease.Parasitology research · 2025Article
- Prevalence and molecular characterization of cystic echinococcosis in livestock in the Hazara Division, Pakistan.Frontiers in veterinary science · 2025Article
- Genetic diversity and population structure ofJournal of advanced veterinary and animal research · 2024Article
- G1 the commonJournal of veterinary science · 2024Article
- A Canadian children's hospital's experience with cystic echinococcosis over 30 years: A case series.Journal of the Association of Medical Microbiology and Infectious Disease Canada = Journal officiel de l'Association pour la microbiologie medicale et l'infectiologie Canada · 2024Article
- Genetic Characterization ofDiseases (Basel, Switzerland) · 2023Article
- Update on the genetic diversity and population structure of Echinococcus granulosus in Gansu Province, Tibet Autonomous Region, and Xinjiang Uygur Autonomous Region, Western China, inferred from mitochondrial cox1, nad1, and nad5 sequences.Parasitology research · 2023Article
- Survey and Molecular Characterization of Echinococcus granulosus sensu stricto from Livestock and Humans in the Altai Region of Xinjiang, ChinaPathogens (Basel, Switzerland) · 2023Article
- Revealing novelFrontiers in veterinary science · 2023Article
- Occurrence and genetic characterization of Echinococcus granulosus sensu lato from domestic animals in Central Iran.BMC veterinary research · 2022Article
- Food-borne zoonotic echinococcosis: A review with special focus on epidemiology.Frontiers in veterinary science · 2022Review
- Genetic diversity and haplotype analysis of yak and sheep echinococcal cysts isolates from the mitochondrialFrontiers in veterinary science · 2022Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundCystic echinococcosis (CE) is a serious tapeworm infection caused by Echinococcus granulosus (sensu lato) which infects a wide range of animals and humans worldwide. Despite the millions of livestock heads reared in Pakistan, only a few reports on CE prevalence and even fewer on the genetic diversity are available for the country. Meanwhile, the available reports on the genetic diversity are predominantly based on short sequences of the cox1 gene.
methodsTo close this knowledge gap, this study was designed to investigate the genetic diversity and population structure of Echinococcus spp. in Pakistan using the complete mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) genes.
resultsBased on BLAST searches of the generated cox1 and nad1 gene sequences from a total of 60 hydatid cysts collected from cattle (n = 40) and buffalo (n = 20), 52 isolates were identified as E. granulosus (s.s.) (G1, G3) and 8 as E. ortleppi (G5). The detection of the G5 genotype represents the first in Pakistan. The phylogeny inferred by the Bayesian method using nucleotide sequences of cox1-nad1 further confirmed their identity. The diversity indices indicated a high haplotype diversity and a low nucleotide diversity. The negative values of Tajima's D and Fu's Fs test demonstrated deviation from neutrality suggesting a recent population expansion.
conclusionsTo the best of our knowledge, this report described the genetic variation of E. granulosus population for the first time in Pakistan using the complete cox1 and nad1 mitochondrial genes and confirms E. ortleppi as one of the causative agents of CE among livestock in Pakistan. While this report will contribute to baseline information for CE control, more studies considering species diversity and distribution in different hosts across unstudied regions of Pakistan are highly needed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.