ArticleInternational journal for parasitology2020
Development of caecaloids to study host-pathogen interactions: new insights into immunoregulatory functions of Trichuris muris extracellular vesicles in the caecum.
Article in International journal for parasitology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Preclinical animal models for onchocerciasis and loiasis: A systematic review of applications in drug screening.PLoS neglected tropical diseases · 2026Pooled it
- Lessons on multicellular two- (2D) and three-dimensional (3D) culture in parasitology: Insights, challenges and future directions.International journal for parasitology · 2026Review
- Development of a novel organoid-based two-dimensional system to model the intestinal host-parasite interface in fasciolosis.Frontiers in cellular and infection microbiology · 2026Article
- Transcriptional responses of mouse proximal colon and colonoids during early whipworm infection.mBio · 2025Article
- Patterned gastrointestinal monolayers with bilateral access as observable models of parasite gut infection.Nature biomedical engineering · 2025Article
- Anisakis extracellular vesicles elicit immunomodulatory and potentially tumorigenic outcomes on human intestinal organoids.Parasites & vectors · 2024Article
- Organoids as a tool to study the impact of heterogeneity in gastrointestinal epithelium on host-pathogen interactions.Clinical and experimental immunology · 2024Review
- Overview of extracellular vesicles in pathogens with special focus on human extracellular protozoan parasites.Memorias do Instituto Oswaldo Cruz · 2024Review
- Extracellular vesicles secreted by Brugia malayi microfilariae modulate the melanization pathway in the mosquito host.Scientific reports · 2023Article
- Whipworm secretions and their roles in host-parasite interactions.Parasites & vectors · 2022Review
- Helminth-induced reprogramming of the stem cell compartment inhibits type 2 immunity.The Journal of experimental medicine · 2022Article
- Review
- Defining the early stages of intestinal colonisation by whipworms.Nature communications · 2022Article
- The Roles of Extracellular Vesicles and Organoid Models in Female Reproductive Physiology.International journal of molecular sciences · 2022Review
- Secreted filarial nematode galectins modulate host immune cells.Frontiers in immunology · 2022Article
- Organoids as tools to investigate gastrointestinal nematode development and host interactions.Frontiers in cellular and infection microbiology · 2022Article
- The interplay betweenParasitology · 2021Article
- Immunoregulatory molecules secreted byParasitology · 2021Article
- Overview of Immunological Responses and Immunomodulation Properties ofLife (Basel, Switzerland) · 2021Review
- Extracellular vesicles from Heligmosomoides bakeri and Trichuris muris contain distinct microRNA families and small RNAs that could underpin different functions in the host.International journal for parasitology · 2020Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
The caecum, an intestinal appendage in the junction of the small and large intestines, displays a unique epithelium that serves as an exclusive niche for a range of pathogens including whipworms (Trichuris spp.). While protocols to grow organoids from small intestine (enteroids) and colon (colonoids) exist, the conditions to culture organoids from the caecum have yet to be described. Here, we report methods to grow, differentiate and characterise mouse adult stem cell-derived caecal organoids, termed caecaloids. We compare the cellular composition of caecaloids with that of enteroids, identifying differences in intestinal epithelial cell populations that mimic those found in the caecum and small intestine. The remarkable similarity in the intestinal epithelial cell composition and spatial conformation of caecaloids and their tissue of origin enables their use as an in vitro model to study host interactions with important caecal pathogens. Thus, exploiting this system, we investigated the responses of caecal intestinal epithelial cells to extracellular vesicles secreted/excreted by the intracellular helminth Trichuris muris. Our findings reveal novel immunoregulatory effects of whipworm extracellular vesicles on the caecal epithelium, including the downregulation of responses to nucleic acid recognition and type-I interferon signalling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.