ArticlePLoS neglected tropical diseases2020
Melioidosis DS rapid test: A standardized serological dipstick assay with increased sensitivity and reliability due to multiplex detection.
Article in PLoS neglected tropical diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Development and international multicenter evaluation of a second-generation immunochromatography test for the serological diagnosis of melioidosis.PLoS neglected tropical diseases · 2026Article
- Article
- Melioidosis in South Asia: The hidden endemicity we can no longer ignore.Current research in microbial sciences · 2026Article
- Serologic Evidence of Exposure to Burkholderia pseudomallei, Nigeria.Emerging infectious diseases · 2026Article
- Laboratory diagnosis of melioidosis.PLoS neglected tropical diseases · 2025Review
- Stability assessment of whole cell ELISA for Burkholderia pseudomallei antibodies detection.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Melioidosis molecular diagnostics: An update.Virulence · 2025Review
- Point-of-care detection of Burkholderia pseudomallei: A method integrating LAMP and LFSA with RNase HII hydrolysis.PLoS neglected tropical diseases · 2025Article
- Melioidosis with venous thrombosis and cellulitis in the left lower limb: a case report.Journal of medical case reports · 2025Article
- Performance of Antibody-Detection Tests for Human Melioidosis: A Systematic Review and Meta-analysis.The Malaysian journal of medical sciences : MJMS · 2024Review
- The hidden burden of melioidosis in Nepal: a paradigm for the urgent need to implement a simple laboratory algorithm to detectIJID regions · 2024Review
- Development of a novel sequence based real-time PCR assay for specific and sensitive detection of Burkholderia pseudomallei in clinical and environmental matrices.Annals of clinical microbiology and antimicrobials · 2024Article
- Isolation of Burkholderia pseudomallei from a goat in New Caledonia: implications for animal and human health monitoring and serological tool comparison.BMC veterinary research · 2024Article
- Burkholderia pseudomallei and melioidosis.Nature reviews. Microbiology · 2024Review
- Protein Microarray-Guided Development of a Highly Sensitive and Specific Dipstick Assay for Glanders Serodiagnostics.Journal of clinical microbiology · 2023Article
- Serodiagnosis of Abdominal Abscess Caused byInfection and drug resistance · 2023Article
- Deciphering the human antibody response againstFrontiers in immunology · 2023Article
- Highly specific and sensitive detection of Burkholderia pseudomallei genomic DNA by CRISPR-Cas12a.PLoS neglected tropical diseases · 2022Article
- A recombinase polymerase amplification lateral flow assay for rapid detection of Burkholderia pseudomallei, the causative agent of melioidosis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- Open-Source, Adaptable, All-in-One Smartphone-Based System for Quantitative Analysis of Point-of-Care Diagnostics.Diagnostics (Basel, Switzerland) · 2022Article
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Authors and funding
19 authors at 7 institutions in 4 countries.
Funding
Abstract
backgroundMelioidosis, caused by Burkholderia pseudomallei, is a severe infectious disease with high mortality rates, but is under-recognized worldwide. In endemic areas, there is a great need for simple, low-cost and rapid diagnostic tools. In a previous study we showed, that a protein multiplex array with 20 B. pseudomallei-specific antigens detects antibodies in melioidosis patients with high sensitivity and specificity. In a subsequent study the high potential of anti-B. pseudomallei antibody detection was confirmed using a rapid Hcp1 single protein-based assay. Our protein array also showed that the antibody profile varies between patients, possibly due to a combination of host factors but also antigen variations in the infecting B. pseudomallei strains. The aim of this study was to develop a rapid test, combining Hcp1 and the best performing antigens BPSL2096, BPSL2697 and BPSS0477 from our previous study, to take advantage of simultaneous antibody detection. METHODS AND PRINCIPAL
findingsThe 4-plex dipstick was validated with sera from 75 patients on admission plus control groups, achieving 92% sensitivity and 97-100% specificity. We then re-evaluated melioidosis sera with the 4-plex assay that were previously misclassified by the monoplex Hcp1 rapid test. 12 out of 55 (21.8%) false-negative samples were positive in our new dipstick assay. Among those, 4 sera (7.3%) were Hcp1 positive, whereas 8 (14.5%) sera remained Hcp1 negative but gave a positive reaction with our additional antigens.
conclusionsOur dipstick rapid test represents an inexpensive, standardized and simple diagnostic tool with an improved serodiagnostic performance due to multiplex detection. Each additional band on the test strip makes a false-positive result more unlikely, contributing to its reliability. Future prospective studies will seek to validate the gain in sensitivity and specificity of our multiplex rapid test approach in different melioidosis patient cohorts.
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