ArticleThe Laryngoscope2021
Restricted Water Intake Adversely Affects Rat Vocal Fold Biology.
Article in The Laryngoscope, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 11 citations in OpenAlex.
- Water limitation modifies vocal performance in California mice: implications for acoustic communication under environmental stress.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2026Article
- Impact of Circulating Estrogen and Mild Systemic Dehydration on Ultrasonic Vocalizations in Female Rats.Journal of speech, language, and hearing research : JSLHR · 2026Article
- Molecular changes, histopathology, and ultrasonic vocalization acoustic profiles of systemically dehydrated rats.PloS one · 2025Article
- Impact of Rehydration Following Systemic Dehydration on Vocal Fold Gene Expression.The Laryngoscope · 2023Article
- Hydration State and Hyaluronidase Treatment Significantly Affect Porcine Vocal Fold Biomechanics.Journal of voice : official journal of the Voice Foundation · 2023Article
- Comparative proteomic changes in rabbit vocal folds undergoing systemic dehydration and systemic rehydration.Journal of proteomics · 2023Article
- The role of systemic dehydration in vocal fold healing: Preliminary findings.Laryngoscope investigative otolaryngology · 2022Article
- Proteomic analysis reveals that aging rabbit vocal folds are more vulnerable to changes caused by systemic dehydration.BMC genomics · 2022Article
- Furosemide-induced systemic dehydration alters the proteome of rabbit vocal folds.Journal of proteomics · 2022Article
- Recurring exposure to low humidity induces transcriptional and protein level changes in the vocal folds of rabbits.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
objectivesA holistic understanding of the many ways that systemic dehydration affects vocal fold biology is still evolving. There are also myriad physiologically relevant methodologies to induce systemic dehydration. To untangle the effects of systemic dehydration on vocal fold biology, we need to utilize realistic, clinically translatable paradigms of systemic dehydration in lab animals. Restricted access to water accommodates clinical translation. We investigated whether systemic dehydration via reduced water intake would negatively affect vocal fold biology. STUDY
designProspective, in vivo study design.
methodsMale Sprague Dawley rats (N = 13) were provided 4 mL/100 g of water/day for 5 days, whereas male control rats (N = 8) were given ad lib access to water. Following euthanasia, tissues were processed for histological staining, gene expression, and protein assays.
resultsRenin gene expression level in kidneys increased significantly (P ≤ .05), validating dehydration. Dehydration induced by restricted water access downregulated the gene expression of interleukin-1α and desmoglein-1 (P ≤ .05). Hyaluronidase-2 gene expression increased after dehydration (P ≤ .05). The protein level of desmoglein-1 decreased after dehydration (P ≤ .05). Histological analyses suggested decreased hyaluronan (P ≤ .05) in the water-restricted rat vocal fold.
conclusionReduced daily water intake for just 5 days impairs vocal fold biology by disrupting inflammatory cytokine release, reducing plasma membrane integrity, and disrupting the hyaluronan network. This is the first study investigating the dehydrating effects of restricted water intake on vocal fold tissue in an in vivo model. LEVEL OF EVIDENCE: NA (prospective animal study). Laryngoscope, 131:839-845, 2021.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.