Evidence map›Paper›PMID 32658266›Full record

ArticleDNA research : an international journal for rapid publication of reports on genes and genomes2020

Platanus_B: an accurate de novo assembler for bacterial genomes using an iterative error-removal process.

Rei Kajitani, Dai Yoshimura, Yoshitoshi Ogura, Yasuhiro Gotoh, Tetsuya Hayashi, Takehiko Itoh

Abstract read
In one paragraph

Article in DNA research : an international journal for rapid publication of reports on genes and genomes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed.

  1. Article
  2. Isolation and genetic analysis of anBiochemistry and biophysics reports · 2026
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  3. Draft genome sequences ofMicrobiology resource announcements · 2026
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  8. Draft genome sequences ofMicrobiology resource announcements · 2025
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  9. Article
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  12. Article
  13. Whole-genome-based characterization ofMicrobial genomics · 2025
    Article
  14. International journal of systematic and evolutionary microbiology · 2025
    Article
  15. Article
  16. Article
  17. Article
  18. Complete genome sequences of 11Microbiology resource announcements · 2024
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Rei KajitaniSchool of Life Science and Technology, Tokyo Institute of Technology, Tokyo 152-8550, Japan.
Dai YoshimuraSchool of Life Science and Technology, Tokyo Institute of Technology, Tokyo 152-8550, Japan.
Yoshitoshi OguraDivision of Microbiology, Department of Infectious Medicine, Kurume University School of Medicine, Kurume, Fukuoka 830-0011, Japan.
Yasuhiro GotohDepartment of Bacteriology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
Tetsuya HayashiDepartment of Bacteriology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
Takehiko ItohSchool of Life Science and Technology, Tokyo Institute of Technology, Tokyo 152-8550, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

De novo assembly of short DNA reads remains an essential technology, especially for large-scale projects and high-resolution variant analyses in epidemiology. However, the existing tools often lack sufficient accuracy required to compare closely related strains. To facilitate such studies on bacterial genomes, we developed Platanus_B, a de novo assembler that employs iterations of multiple error-removal algorithms. The benchmarks demonstrated the superior accuracy and high contiguity of Platanus_B, in addition to its ability to enhance the hybrid assembly of both short and nanopore long reads. Although the hybrid strategies for short and long reads were effective in achieving near full-length genomes, we found that short-read-only assemblies generated with Platanus_B were sufficient to obtain ≥90% of exact coding sequences in most cases. In addition, while nanopore long-read-only assemblies lacked fine-scale accuracies, inclusion of short reads was effective in improving the accuracies. Platanus_B can, therefore, be used for comprehensive genomic surveillances of bacterial pathogens and high-resolution phylogenomic analyses of a wide range of bacteria.

Indexed as

AlgorithmsGenome, BacterialBacteriaDNA, BacterialEscherichia coliGenomicsPhylogenyDNA, BacterialBacterial genomede novo assemblyhigh-resolution phylogenomicslarge-scale genomic surveillance

Identifiers

PMID32658266
PMCPMC7433917

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.