Evidence map›Paper›PMID 32658202›Full record

ArticleJournal of visualized experiments : JoVE2020

Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis.

Wei Liao, Chaoyu Ma, Nu Zhang

Abstract readVideo-Audio Media
In one paragraph

Article in Journal of visualized experiments : JoVE, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Wei LiaoDepartment of Microbiology, Immunology and Molecular Genetics, School of Medicine, University of Texas Health Science Center at San Antonio; Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital, Central South University.
Chaoyu MaDepartment of Microbiology, Immunology and Molecular Genetics, School of Medicine, University of Texas Health Science Center at San Antonio.
Nu ZhangDepartment of Microbiology, Immunology and Molecular Genetics, School of Medicine, University of Texas Health Science Center at San Antonio; ZhangN3@uthscsa.edu.

Funding

TISSUE CULTURE---COREP30CA054174 · NCI · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI Lei Zheng · 1991 to 2026
$59.1M
Institute for Integration of Medicine & Science: A Partnership to Improve HealthUL1TR001120 · NCATS · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI CLARK, ROBERT A, HARGREAVES, KENNETH M · 2013 to 2017
$16.5M
Mechanistic study of TGF-beta-dependent control of gut-resident memory T cellsR01AI125701 · NIAID · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI ZHANG, NU · 2016 to 2020
$2.0M
Mechanistic studies of Tissue-resident T cell maturation in the kidneyR21AI139721 · NIAID · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI ZHANG, NU · 2018 to 2019
$419k
NCATS NIH HHS UL1 TR001120NCI NIH HHS P30 CA054174NIAID NIH HHS R01 AI125701NIAID NIH HHS R21 AI139721
6 · The paper itself

Abstract

Tissue-resident memory T cell (TRM) is a rapidly expanding field of immunology research. Isolating T cells from various non-lymphoid tissues is one of the key steps to investigate TRMs. There are slight variations in lymphocyte isolation protocols for different organs. Kidney is an essential non-lymphoid organ with numerous immune cell infiltration especially after pathogen exposure or autoimmune activation. In recent years, multiple labs including our own have started characterizing kidney resident CD8

Indexed as

Flow CytometryAnimalsCD8-Positive T-LymphocytesDisease Models, AnimalImmunologic MemoryKidneyLymphocytic ChoriomeningitisLymphocytic choriomeningitis virusMiceMice, Inbred C57BL

Identifiers

PMID32658202
PMCPMC8493567

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.