ArticlePlant molecular biology2020
Comparative analysis of the transcriptome, methylome, and metabolome during pollen abortion of a seedless citrus mutant.
Article in Plant molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 22 citations in OpenAlex.
- Comparative gametogenesis and genomic signatures associated with pollen sterility in the seedless mutant of grapevine.BMC plant biology · 2025Article
- Silencing of a glycosyltransferase-like protein in citrus reduces male and female fertility impacting seed development in self-pollinated fruit.Frontiers in plant science · 2025Article
- Gene expression profiles and metabolic pathways responsible for male sterility in cybrid pummelo.Plant cell reports · 2024Article
- Transcription Factors and Their Regulatory Roles in the Male Gametophyte Development of Flowering Plants.International journal of molecular sciences · 2024Review
- Volatile metabolomics and transcriptomics analyses provide insights into the mechanism of volatile changes during fruit development of 'Ehime 38' (Frontiers in plant science · 2024Article
- Article
- Biosynthetic regulatory network of flavonoid metabolites in stems and leaves of Salvia miltiorrhiza.Scientific reports · 2022Article
- Pan-mitogenomics reveals the genetic basis of cytonuclear conflicts in citrus hybridization, domestication, and diversification.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Mitogen-activated protein kinase 4 is obligatory for late pollen and early fruit development in tomato.Horticulture research · 2022Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
key messagePollen abortion could be mainly attributed to abnormal meiosis in the mutant. Multiomics analysis uncovered significant epigenetic variations between the mutant and its wild type during the pollen abortion process. Male sterility caused by aborted pollen can result in seedless fruit. A seedless Ponkan mandarin mutant (bud sport) was used to compare the transcriptome, methylome, and metabolome with its progenitor to understand the mechanism of citrus pollen abortion. Cytological observations showed that the anther of the mutant could form microspore mother cells, although the microspores failed to develop fertile pollen at the anther dehiscence stage. Based on pollen phenotypic analysis, pollen abortion could be mainly attributed to abnormal meiosis in the mutant. A transcriptome analysis uncovered the molecular mechanisms underlying pollen abortion between the mutant and its wild type. A total of 5421 differentially expressed genes were identified, and some of these genes were involved in the meiosis, hormone biosynthesis and signaling, carbohydrate, and flavonoid pathways. A total of 50,845 differentially methylated regions corresponding to 15,426 differentially methylated genes in the genic region were found between the mutant and its wild type by the methylome analysis. The expression level of these genes was negatively correlated with their methylation level, especially in the promoter regions. In addition, 197 differential metabolites were identified between the mutant and its wild type based on the metabolome analysis. The transcription and metabolome analysis further indicated that the expression of genes in the flavonoid, carbohydrate, and hormone metabolic pathways was significantly modulated in the pollen of the mutant. These results indicated that demethylation may alleviate the silencing of carbohydrate genes in the mutant, resulting in excessive starch and sugar hydrolysis and thereby causing pollen abortion in the mutant.
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