Evidence map›Paper›PMID 32656578›Full record

ArticleJournal of molecular histology2020

Exosomes derived from human exfoliated deciduous teeth ameliorate adult bone loss in mice through promoting osteogenesis.

Jizhen Wei, Yeqing Song, Zhihao Du, Feiyan Yu, Yimei Zhang, Nan Jiang, Xuejun Ge

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Journal of molecular histology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 50 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
50citing papers in PubMed, 4 pooled it
3.4field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

50 citing papers in PubMed, 4 syntheses or guidelines pooled it, 82 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
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  4. Pooled it
  5. Article
  6. Article
  7. Article
  8. Review
  9. Isolation methods of exosomes derived from dental stem cells.International journal of oral science · 2025
    Review
  10. Article
  11. Review
  12. Review
  13. Review
  14. Mesenchymal stem cell-derived extracellular vesicles in periodontal bone repair.Journal of molecular medicine (Berlin, Germany) · 2025
    Review
  15. Review
  16. Review
  17. Article
  18. Article
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  20. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Jizhen WeiDepartment of Periodontics, Shanxi Medical University School and Hospital of Stomatology, Taiyuan, Shanxi, China.
Yeqing SongCentral Laboratory, Peking University School and Hospital of Stomatology, #22 Zhongguancun South Avenue, Haidian District, Beijing, 100081, China.
Zhihao DuCentral Laboratory, Peking University School and Hospital of Stomatology, #22 Zhongguancun South Avenue, Haidian District, Beijing, 100081, China.
Feiyan YuDepartment of Oral Medicine, Shanxi Medical University School and Hospital of Stomatology, Taiyuan, Shanxi, China.
Yimei ZhangFirst Dental Center, Peking University School and Hospital of Stomatology, Beijing, China.
Nan JiangCentral Laboratory, Peking University School and Hospital of Stomatology, #22 Zhongguancun South Avenue, Haidian District, Beijing, 100081, China. nanjiang@bjmu.edu.cn.ORCID http://orcid.org/0000-0002-8408-359X
Xuejun GeDepartment of Periodontics, Shanxi Medical University School and Hospital of Stomatology, Taiyuan, Shanxi, China. gxj19722003@163.com.
Peking University · CNShanxi Medical University · CNKing University · US

Funding

National Natural Science Foundation of China 81600820National Natural Science Foundation of China 81801014National Natural Science Foundation of China 81970901
6 · The paper itself

Abstract

Cell-free based therapy is an effective strategy in regenerative medicine as it avoids controversial issues, such as immunomodulation and stability. Recently, exosomes have been explored as a favorable substitution for stem cell therapy as they exhibit multiple advantages, such as the ability to be endocytosed and innate biocompatibility. This study aimed to investigate the effects of stem cells from human exfoliated deciduous teeth (SHED)-derived exosomes (SHED-Exo) on bone marrow stromal cells (BMSCs) osteogenesis and bone recovery. SHED-Exo were isolated, characterized, and applied to the bone loss area caused by periodontitis in a mouse model. We found that the injection of SHED-Exo restored bone loss to the same extent as original stem cells. Without affecting BMSCs proliferation, SHED-Exo mildly inhibited apoptosis. Moreover, SHED-Exo specifically promoted BMSCs osteogenesis and inhibited adipogenesis compared with SHED-derived conditioned medium. The expression of osteogenic marker genes, alkaline phosphatase activity, and Alizarin Red S staining of BMSCs was significantly increased by co-culturing with SHED-Exo. Moreover, Western blot analysis showed that Runx2, a key transcriptional factor in osteogenic differentiation, and p-Smad5 were upregulated upon SHED-Exo stimulation. Expression of the adipogenic marker PPARγ and the amount of lipid droplets decreased when exosomes were present. Low doses of exosomes inhibited the expression of the inflammatory cytokines IL-6 and TNF-α. In conclusion, SHED-Exo directly promoted BMSCs osteogenesis, differentiation, and bone formation. Therefore, exosomes have the potential to be utilized in the treatment of periodontitis and other bone diseases.

Indexed as

AdipogenesisAlkaline PhosphataseAnimalsBiomarkersBone ResorptionCell DifferentiationCell ProliferationCells, CulturedExosomesGene Expression RegulationHumansMaleMesenchymal Stem CellsMiceOsteogenesisStem CellsAlkaline PhosphataseBiomarkersBone lossConditioned mediumExosomesOsteogenesisStem cells from human exfoliated deciduous teeth (SHED)

Identifiers

PMID32656578
OpenAlexW3041277952

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.