Evidence map›Paper›PMID 32655505›Full record

ArticleFrontiers in microbiology2020

Characterization of Bacterial and Fungal Assemblages From Historically Contaminated Metalliferous Soils Using Metagenomics Coupled With Diffusion Chambers and Microbial Traps.

Ashish Pathak, Rajneesh Jaswal, Xiaoyu Xu, John R White, Bobby Edwards, Jaden Hunt, Scott Brooks, Rajesh Singh Rathore, Meenakshi Agarwal, Ashvini Chauhan

Open access · goldAbstract read
In one paragraph

Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Shotgun metagenomics analysis indicatesMicrobiology resource announcements · 2024
    Article
  7. The InvasivePlants (Basel, Switzerland) · 2023
    Article
  8. Review
  9. Friend or Foe: Draft Genome Sequence ofMicrobiology resource announcements · 2022
    Article
  10. Review
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Ashish PathakSchool of the Environment, Florida A&M University, Tallahassee, FL, United States.
Rajneesh JaswalSchool of the Environment, Florida A&M University, Tallahassee, FL, United States.
Xiaoyu XuSavannah River Ecology Laboratory, University of Georgia, Aiken, SC, United States.
John R WhiteDepartment of Oceanography and Coastal Sciences, Louisiana State University, Baton Rouge, LA, United States.
Bobby EdwardsSchool of the Environment, Florida A&M University, Tallahassee, FL, United States.
Jaden HuntSchool of the Environment, Florida A&M University, Tallahassee, FL, United States.
Scott BrooksEnvironmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.
Rajesh Singh RathoreSchool of the Environment, Florida A&M University, Tallahassee, FL, United States.
Meenakshi AgarwalSchool of the Environment, Florida A&M University, Tallahassee, FL, United States.
Ashvini ChauhanSchool of the Environment, Florida A&M University, Tallahassee, FL, United States.
Florida Agricultural and Mechanical University · USLouisiana State University · USOak Ridge National Laboratory · USUniversity of Georgia · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The majority of environmental microbiomes are not amenable to cultivation under standard laboratory growth conditions and hence remain uncharacterized. For environmental applications, such as bioremediation, it is necessary to isolate microbes performing the desired function, which may not necessarily be the fast growing or the copiotroph microbiota. Toward this end, cultivation and isolation of microbial strains using diffusion chambers (DC) and/or microbial traps (MT) have both been recently demonstrated to be effective strategies because microbial enrichment is facilitated by soil nutrients and not by synthetically defined media, thus simulating their native habitat. In this study, DC/MT chambers were established using soils collected from two US Department of Energy (DOE) sites with long-term history of heavy metal contamination, including mercury (Hg). To characterize the contamination levels and nutrient status, soils were first analyzed for total mercury (THg), methylmercury (MeHg), total carbon (TC), total nitrogen (TN), and total phosphorus (TP). Multivariate statistical analysis on these measurements facilitated binning of soils under high, medium and low levels of contamination. Bacterial and fungal microbiomes that developed within the DC and MT chambers were evaluated using comparative metagenomics, revealing

Indexed as

culturomicsdiffusion chambermercury resistancemetagenomicsmicrobial trap

Identifiers

PMID32655505
PMCPMC7325934
OpenAlexW3034283924

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.