ArticlePlant reproduction2020
Live Imaging of embryogenic structures in Brassica napus microspore embryo cultures highlights the developmental plasticity of induced totipotent cells.
Article in Plant reproduction, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- From pollen precursor to totipotent embryo: a model system for plant cell developmental plasticity.Plant cell reports · 2026Review
- Unresolved ER stress restricts in vitro plant cell totipotency.Plant cell reports · 2025Article
- Modulation of calcium, callose synthesis, membrane permeability and pectin methyl-esterase activity affect cell wall composition and embryo yield during Brassica napus microspore embryogenesis.Annals of botany · 2025Article
- Callus Culture System fromInternational journal of molecular sciences · 2025Article
- Establishment and maintenance of embryogenic cell fate during microspore embryogenesis.The Plant journal : for cell and molecular biology · 2025Article
- Analysis of auxin responses in the fern Ceratopteris richardii identifies the developmental phase as a major determinant for response properties.Development (Cambridge, England) · 2024Article
- The maternal embrace: the protection of plant embryos.Journal of experimental botany · 2024Review
- New Epigenetic Modifier Inhibitors Enhance Microspore Embryogenesis in Bread Wheat.Plants (Basel, Switzerland) · 2024Article
- Calcium levels modulate embryo yield inFrontiers in plant science · 2024Article
- The effect of phytosulfokine alpha on haploid embryogenesis and gene expression ofFrontiers in plant science · 2024Article
- Review
- The Highly EmbryogenicPlants (Basel, Switzerland) · 2023Article
- Microtubule organization changes severely after mannitol and n-butanol treatments inducing microspore embryogenesis in bread wheat.BMC plant biology · 2021Article
- Embryogenesis of European Radish (Plants (Basel, Switzerland) · 2021Article
- Cell Wall Composition and Structure Define the Developmental Fate of Embryogenic Microspores inFrontiers in plant science · 2021Article
- Differential Expression Profiling Reveals Stress-Induced Cell Fate Divergence in Soybean Microspores.Plants (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
key messageIn vitro embryo development is highly plastic; embryo cell fate can be re-established in tissue culture through different pathways. In most angiosperms, embryo development from the single-celled zygote follows a defined pattern of cell divisions in which apical (embryo proper) and basal (root and suspensor) cell fates are established within the first cell divisions. By contrast, embryos that are induced in vitro in the absence of fertilization show a less regular initial cell division pattern yet develop into histodifferentiated embryos that can be converted into seedlings. We used the Brassica napus microspore embryogenesis system, in which the male gametophyte is reprogrammed in vitro to form haploid embryos, to identify the developmental fates of the different types of embryogenic structures found in culture. Using time-lapse imaging of LEAFY COTYLEDON1-expressing cells, we show that embryogenic cell clusters with very different morphologies are able to form haploid embryos. The timing of surrounding pollen wall (exine) rupture is a major determinant of cell fate in these clusters, with early exine rupture leading to the formation of suspensor-bearing embryos and late rupture to suspensorless embryos. In addition, we show that embryogenic callus, which develops into suspensor-bearing embryos, initially expresses transcripts associated with both basal- and apical-embryo cell fates, suggesting that these two cell fates are fixed later in development. This study reveals the inherent plasticity of in vitro embryo development and identifies new pathways by which embryo cell fate can be established.
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Registered trials
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