ArticleJournal of assisted reproduction and genetics2020
Altered expression of activator proteins that control follicle reserve after ovarian tissue cryopreservation/transplantation and primordial follicle loss prevention by rapamycin.
Article in Journal of assisted reproduction and genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Rapamycin preserves primordial follicles during closed‑system vitrification of mouse ovarian tissue.Clinical and experimental reproductive medicine · 2026Article
- Surgical approaches to fertility preservation in the cancer patient: current and future directions.Frontiers in endocrinology · 2026Review
- Pituitary extract supplementation enhances follicular survival and gonadotropin receptor expression in vitrified ovarian tissue of Aceh cattle, Indonesia.Veterinary world · 2025Article
- The mechanism and protective strategies of follicle injury after ovarian tissue cryopreservation and thawed transplantation: a review.Journal of ovarian research · 2025Review
- Ovarian cryopreservation with rapamycin improves fertility restoration in a murine orthotopic transplantation model.Scientific reports · 2025Article
- Luteinizing Hormone Receptor Mutation (LHRInterdisciplinary sciences, computational life sciences · 2024Article
- mTOR inhibitors potentially preserve fertility in female patients with haematopoietic malignancies: a narrative review.Annals of hematology · 2024Review
- Uncovering the Penile Clock: Expression of Molecular Clock Proteins in Human Penile Cavernous Tissue.The world journal of men's health · 2024Article
- Review
- Review
- The mTOR Inhibitor Rapamycin Counteracts Follicle Activation Induced by Ovarian Cryopreservation in Murine Transplantation Models.Medicina (Kaunas, Lithuania) · 2023Article
- Recombinant anti-Mullerian hormone treatment attenuates primordial follicle loss after ovarian cryopreservation and transplantation.Journal of assisted reproduction and genetics · 2023Article
- Cancer Treatment-Related Ovarian Dysfunction in Women of Childbearing Potential: Management and Fertility Preservation Options.Journal of clinical oncology : official journal of the American Society of Clinical Oncology · 2023Review
- Impacts of vitrification on the transcriptome of human ovarian tissue in patients with gynecological cancer.Frontiers in genetics · 2023Article
- Rapamycin maintains the primordial follicle pool and protects ovarian reserve against cyclophosphamide-induced damage.The Journal of reproduction and development · 2022Article
- Expression of pro-apoptotic and anti-apoptotic proteins in granulosa cells of women with diminished ovarian reserve.Journal of assisted reproduction and genetics · 2022Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
purposeWe investigated whether expression of activator proteins that control follicle reserve and growth change after ovarian tissue vitrification and re-transplantation. Moreover, we assessed whether inhibition of mTOR signaling pathway by rapamycin would protect primordial follicle reserve after ovarian tissue freezing/thawing and re-transplantation.
methodsFresh control, frozen/thawed, fresh-transplanted, frozen/thawed and transplanted, rapamycin/control, rapamycin fresh-transplanted, and rapamycin frozen-thawed and transplanted groups were established in rats. After freezing and thawing process, two ovaries were transplanted into the back muscle of the same rat. After 2 weeks, grafts were harvested, fixed, and embedded into paraffin block. Normal and atretic primordial/growing follicle count was performed in all groups. Ovarian tissues were evaluated for the dynamic expressions of Gdf-9, Bmp-15, KitL, Lif, Fgf-2, and p-s6K using immunohistochemistry, and H-score analyses were done.
resultsPrimordial follicle reserve reduced almost 50% after ovarian tissue re-transplantation. Expression of Gdf-9 and Lif increased significantly in primordial and growing follicles in frozen-thawed, fresh-transplanted, and frozen/thawed and transplanted groups, whereas expression of Bmp-15, KitL, and Fgf-2 decreased in primordial follicles. Freezing and thawing of ovarian tissue solely significantly increased p-s6K expression in primordial follicles, and on the other hand, suppression of mTORC1 pathway using rapamycin preserved the primordial follicle pool.
conclusionAltered expressions of activator proteins that regulate primordial follicle reserve and growth may lead to primordial follicle loss and rapamycin treatment can protect ovarian reserve after ovarian tissue cryopreservation/transplantation.
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