ArticleClinical oral investigations2021
Microstructural composition, ion release, and bioactive potential of new premixed calcium silicate-based endodontic sealers indicated for warm vertical compaction technique.
Article in Clinical oral investigations, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- Effects of resveratrol-enriched endodontic sealants on pulp stem cell viability and collagen production.Acta odontologica latinoamericana : AOL · 2026Article
- Osteogenic Potential of Various Premixed Hydraulic Calcium Silicate-Based Sealers on Human Bone Marrow Stem Cells.Materials (Basel, Switzerland) · 2025Article
- Antimicrobial, toxicity, and cellular interactions of bioceramic sealers in periodontal ligament: in vitro insights.Clinical oral investigations · 2025Article
- Microstructural and Elemental Characterization of Calcium Silicate-Based Sealers.Nanomaterials (Basel, Switzerland) · 2025Article
- Effect of Bio MTA plus & ProRoot MTA pulp capping materials on the regenerative properties of human dental pulp stem cells.Scientific reports · 2025Article
- Oxidative damage analysis and cell viability of Drosophila melanogaster exposed to three different endodontic sealers: an in vivo and ex vivo study.Odontology · 2024Article
- Effect of the filling technique on the filling removal from oval-shaped canals.Clinical oral investigations · 2024Article
- Premixed calcium silicate-based root canal sealers have better biological properties than AH Plus: A systematic review and meta-analysis of in vivo animal studies andJournal of conservative dentistry and endodontics · 2024Review
- Cytotoxicity and genotoxicity of bioceramic root canal sealers compared to conventional resin-based sealer.Scientific reports · 2024Article
- Biological Properties of Bioceramic Sealers on Osteoblastic Cells: A Comparative Study.Brazilian dental journal · 2024Article
- A comparative study of biological properties of three root canal sealers.Clinical oral investigations · 2023Article
- Advances and challenges in regenerative dentistry: A systematic review of calcium phosphate and silicate-based materials on human dental pulp stem cells.Materials today. Bio · 2023Review
- Influence of bioceramic cones on the quality of root canal filling relative to bond strength and adaptation of the adhesive interface.Clinical oral investigations · 2023Article
- Biocompatibility of three different root canal sealers, experimental study.BMC oral health · 2023Article
- Effect of Different Sealers on the Cytocompatibility and Osteogenic Potential of Human Periodontal Ligament Stem Cells: An In Vitro Study.Journal of clinical medicine · 2023Article
- Effect of obturation technique on penetration of calcium silicate-based sealer into dentinal tubules after endodontic retreatment of mandibular premolars.Clinical oral investigations · 2022Article
- Bioactivity Potential of Bioceramic-Based Root Canal Sealers: A Scoping Review.Life (Basel, Switzerland) · 2022Article
- Are Endodontic Solvents Cytotoxic? An In Vitro Study on Human Periodontal Ligament Stem Cells.Pharmaceutics · 2022Article
- Cytocompatibility and bioactive potential of AH Plus Bioceramic Sealer: An in vitro study.International endodontic journal · 2022Article
- Biocompatibility of a New Calcium Silicate-Based Root Canal Sealer Mediated via the Modulation of Macrophage Polarization in a Rat Model.Materials (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
objectiveThe aim of this study was to evaluate the microstructural composition, ion release, cytocompatibility, and mineralization potential of Bio-C Sealer ION+ (BCI) and EndoSequence BC Sealer HiFlow (BCHiF), compared with AH Plus (AHP), in contact with human periodontal ligament cells (hPDLCs). MATERIALS AND
methodsThe sealers' ionic composition and release were assessed using energy-dispersive spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS), respectively. For the biological assays, hPDLCs were isolated from third molars, and sealer extracts were prepared (undiluted, 1:2, and 1:4 ratios). An MTT assay, wound-healing assay, and cell morphology and adhesion analysis were performed. Activity-related gene expression was determined using RT-qPCR, and mineralization potential was assessed using Alizarin Red staining (ARS). Statistical analyses were performed using one-way ANOVA and Tukey's post hoc test (α < 0.05).
resultsThe three sealers exhibited variable levels of silicon, calcium, zirconium, and tungsten release and in their composition. Both BCI and BCHiF groups showed positive results in cytocompatibility assays, unlike AHP. The BCHiF group showed an upregulation of CAP (p < 0.01), CEMP1, ALP, and RUNX2 (p < 0.001) compared with the negative control, while the BCI group showed an upregulation of CEMP1 (p < 0.01), CAP, and RUNX2 (p < 0.001). Both groups also exhibited a greater mineralization potential than the negative and positive controls (p < 0.001).
conclusionsThe calcium silicate-based sealers considered in the present in vitro study exhibited a high calcium ion release, adequate cytocompatibility, upregulated osteo/cementogenic gene expression, and increased mineralized nodule formation in contact with hPDLCs. CLINICAL RELEVANCE: From a biological perspective, BCI and BCHiF could be clinically suitable for root canal filling.
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Registered trials
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