Evidence map›Paper›PMID 32651372›Full record

ArticleNature communications2020

Mechano-modulatory synthetic niches for liver organoid derivation.

Giovanni Sorrentino, Saba Rezakhani, Ece Yildiz, Sandro Nuciforo, Markus H Heim, Matthias P Lutolf, Kristina Schoonjans

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 107 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
107citing papers in PubMed, 1 pooled it
14.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

107 citing papers in PubMed, 1 synthesis or guideline pooled it, 207 citations in OpenAlex.

  1. Pooled it
  2. Review
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  5. The Application of 3D Cell Culture in Thyroid Cancer.Annals of biomedical engineering · 2026
    Review
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  7. New developments and applications of human organoids.Nature reviews. Molecular cell biology · 2026
    Review
  8. Review
  9. Article
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  16. Article
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  20. Rethinking Matrigel: The Complex Journey to Matrix Alternatives in Organoid Culture.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Review

47 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Giovanni Sorrentino *Laboratory of Metabolic Signaling, Institute of Bioengineering, School of Life Sciences and School of Engineering, Ecole Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland.
Saba Rezakhani *Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences and School of Engineering, École Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.
Ece YildizLaboratory of Metabolic Signaling, Institute of Bioengineering, School of Life Sciences and School of Engineering, Ecole Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland.
Sandro NuciforoDepartment of Biomedicine, University Hospital Basel, University of Basel, 4031, Basel, Switzerland.ORCID http://orcid.org/0000-0002-1863-1869
Markus H HeimDepartment of Biomedicine, University Hospital Basel, University of Basel, 4031, Basel, Switzerland.ORCID http://orcid.org/0000-0002-7523-4894
Matthias P LutolfLaboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences and School of Engineering, École Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland. matthias.lutolf@epfl.ch.ORCID http://orcid.org/0000-0002-5898-305X
Kristina SchoonjansLaboratory of Metabolic Signaling, Institute of Bioengineering, School of Life Sciences and School of Engineering, Ecole Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland. kristina.schoonjans@epfl.ch.ORCID http://orcid.org/0000-0003-1247-4265
École Polytechnique Fédérale de Lausanne · CHUniversity of Basel · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The recent demonstration that primary cells from the liver can be expanded in vitro as organoids holds enormous promise for regenerative medicine and disease modelling. The use of three-dimensional (3D) cultures based on ill-defined and potentially immunogenic matrices, however, hampers the translation of liver organoid technology into real-life applications. We here use chemically defined hydrogels for the efficient derivation of both mouse and human hepatic organoids. Organoid growth is found to be highly stiffness-sensitive, a mechanism independent of acto-myosin contractility and requiring instead activation of the Src family of kinases (SFKs) and yes-associated protein 1 (YAP). Aberrant matrix stiffness, on the other hand, results in compromised proliferative capacity. Finally, we demonstrate the establishment of biopsy-derived human liver organoids without the use of animal components at any step of the process. Our approach thus opens up exciting perspectives for the establishment of protocols for liver organoid-based regenerative medicine.

Indexed as

HumansHydrogelsLiverOrganoidssrc-Family KinasesTissue EngineeringTranscription FactorsHydrogelssrc-Family KinasesTranscription Factors

Identifiers

PMID32651372
PMCPMC7351772
OpenAlexW3041739049

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.