Evidence map›Paper›PMID 32650810›Full record

ArticleBMC research notes2020

Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast.

William T Molin, Allison Yaguchi, Mark Blenner, Christopher A Saski

Open access · goldAbstract read
In one paragraph

Article in BMC research notes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 23 citations in OpenAlex.

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  14. Extrachromosomal Circular DNA (eccDNA): From Chaos to Function.Frontiers in cell and developmental biology · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

William T MolinCrop Production Systems Research Unit, USDA-ARS, Stoneville, MS, 38776, USA.
Allison YaguchiDepartment of Chemical and Biomolecular Engineering, Clemson University, Clemson, SC, 29634, USA.
Mark BlennerDepartment of Chemical and Biomolecular Engineering, Clemson University, Clemson, SC, 29634, USA.
Christopher A SaskiDepartment of Plant and Environmental Sciences, Clemson University, Clemson, SC, 29634, USA. saski@clemson.edu.ORCID http://orcid.org/0000-0002-2780-4274
Clemson University · USAgricultural Research Service · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThe objective of the research presented here was to determine whether autonomous replication sequences (ARS) discovered in the eccDNA replicon of glyphosate resistant Amaranthus palmeri enable self-replication in a yeast system.

resultsSequence analysis of the eccDNA replicon revealed a region of sharp changes in A + T/G + C content with characteristic bending indicative of an autonomous replication sequence. Further sequence analysis revealed an extended autonomous replication sequence (EACS) in close proximity to multiple DNA unwinding element (DUE) sequences. This region of the eccDNA replicon enabled autonomous replication of an ARS-less yeast plasmid.

Indexed as

DNA ReplicationRepliconAmaranthusBase CompositionCloning, MolecularDNA, PlantNucleic Acid ConformationSaccharomyces cerevisiaeSequence Analysis, DNADNA, PlantAutonomous replicationDNA bendingEACSeccDNA replicon

Identifiers

PMID32650810
PMCPMC7350638
OpenAlexW3041205178

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.