ArticleInternational journal of molecular sciences2020
Physiological Importance of Pectin Modifying Genes During Rice Pollen Development.
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Integrative sequence-structure analysis reveals hidden WD40 domains forming stable β-propeller folds with potential biological functions in plants.Plant communications · 2026Article
- β-Xylosidase Overexpression Alters Pectin and Cellulose Distribution and Modulates Blast Disease Resistance in Rice.Plants (Basel, Switzerland) · 2026Article
- Evaluating Pollen Viability and Tube Growth in Rice (Oryza sativa).Methods in molecular biology (Clifton, N.J.) · 2026Article
- Analysis of Genetic Variation of Rice Straw Characteristics and Its Influence on Biomass.Plant direct · 2026Article
- Pectin methylesterase inhibitor 58 negatively regulates ray petal elongation by inhibiting cell expansion in Gerbera hybrida.Plant cell reports · 2025Article
- The Rice Online Expression Profiles Array Database Version 2 (ROADv2): An Interactive Atlas for Rice Functional Genomics.Rice (New York, N.Y.) · 2024Article
- Heterogeneity in Mechanical Properties of Plant Cell Walls.Plants (Basel, Switzerland) · 2024Review
- Transcriptome profiling of foxtail millet (Setaria italica) pollen and anther.BMC plant biology · 2024Article
- Research overview on the genetic mechanism underlying the biosynthesis of polysaccharide in tuber plants.PeerJ · 2024Review
- Acetylation of inorganic pyrophosphatase by S-RNase signaling induces pollen tube tip swelling by repressing pectin methylesterase.The Plant cell · 2023Article
- Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility inInternational journal of molecular sciences · 2023Article
- CRISPR/Cas9 Mutant RiceInternational journal of molecular sciences · 2022Article
- Transcriptome Analysis of Triple Mutant for OsMADS62, OsMADS63, and OsMADS68 Reveals the Downstream Regulatory Mechanism for Pollen Germination in Rice (International journal of molecular sciences · 2021Article
- Genome-wide identification of PME genes, evolution and expression analyses in soybean (Glycine max L.).BMC plant biology · 2021Article
- Article
- Global Identification ofFrontiers in plant science · 2021Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Although cell wall dynamics, particularly modification of homogalacturonan (HGA, a major component of pectin) during pollen tube growth, have been extensively studied in dicot plants, little is known about how modification of the pollen tube cell wall regulates growth in monocot plants. In this study, we assessed the role of HGA modification during elongation of the rice pollen tube by adding a pectin methylesterase (PME) enzyme or a PME-inhibiting catechin extract (Polyphenon 60) to in vitro germination medium. Both treatments led to a severe decrease in the pollen germination rate and elongation. Furthermore, using monoclonal antibodies toward methyl-esterified and de-esterified HGA epitopes, it was found that exogenous treatment of PME and Polyphenon 60 resulted in the disruption of the distribution patterns of low- and high-methylesterified pectins upon pollen germination and during pollen tube elongation. Eleven PMEs and 13 PME inhibitors (PMEIs) were identified by publicly available transcriptome datasets and their specific expression was validated by qRT-PCR. Enzyme activity assays and subcellular localization using a heterologous expression system in tobacco leaves demonstrated that some of the pollen-specific PMEs and PMEIs possessed distinct enzymatic activities and targeted either the cell wall or other compartments. Taken together, our findings are the first line of evidence showing the essentiality of HGA methyl-esterification status during the germination and elongation of pollen tubes in rice, which is primarily governed by the fine-tuning of PME and PMEI activities.
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