ArticleScientific reports2020
Visualization of cytoplasmic organelles via in-resin CLEM using an osmium-resistant far-red protein.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Introduction to the CLEM technique developed in the field of neuroanatomy.Anatomical science international · 2025Article
- α-Synuclein-Assembled Synaptic Vesicle Pools at the Presynaptic Terminal: A Study of α-Synuclein Function Using a Novel Mouse Model.Acta histochemica et cytochemica · 2025Review
- A highly stable monomeric red fluorescent protein for advanced microscopy.Nature methods · 2025Article
- Far-Red Fluorescent Proteins: Tools for Advancing In Vivo Imaging.Biosensors · 2024Review
- Article
- Application of immuno- and affinity labeling with fluorescent dyes to in-resin CLEM of Epon-embedded cells.Heliyon · 2023Article
- In-resin CLEM of Epon-embedded cells using proximity labeling.Scientific reports · 2022Article
- Article
- Multi-scale light microscopy/electron microscopy neuronal imaging from brain to synapse with a tissue clearing method, ScaiScience · 2022Article
- Correlative Organelle Microscopy: Fluorescence Guided Volume Electron Microscopy of Intracellular Processes.Frontiers in cell and developmental biology · 2022Article
- Recent technological advances in correlative light and electron microscopy for the comprehensive analysis of neural circuits.Frontiers in neuroanatomy · 2022Review
- Membranous Structures Directly Come in Contact With p62/SQSTM1 Bodies.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2021Article
- Correlation of organelle dynamics between light microscopic live imaging and electron microscopic 3D architecture using FIB-SEM.Microscopy (Oxford, England) · 2021Review
- A Compartmentalized Neuronal Cell-Culture Platform Compatible With Cryo-Fixation by High-Pressure Freezing for Ultrastructural Imaging.Frontiers in neuroscience · 2021Article
- Two-color in-resin CLEM of Epon-embedded cells using osmium resistant green and red fluorescent proteins.Scientific reports · 2020Article
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Authors and funding
4 authors.
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Abstract
Post-fixation with osmium tetroxide staining and the embedding of Epon are robust and essential treatments that are used to preserve and visualize intracellular membranous structures during electron microscopic analyses. These treatments, however, can significantly diminish the fluorescent intensity of most fluorescent proteins in cells, which creates an obstacle for the in-resin correlative light-electron microscopy (CLEM) of Epon-embedded cells. In this study, we used a far-red fluorescent protein that retains fluorescence after osmium staining and Epon embedding to perform an in-resin CLEM of Epon-embedded samples. The fluorescence of this protein was detected in 100 nm thin sections of the cells in Epon-embedded samples after fixation with 2.5% glutaraldehyde and post-fixation with 1% osmium tetroxide. We performed in-resin CLEM of the mitochondria in Epon-embedded cells using a mitochondria-localized fluorescent protein. Using this protein, we achieved in-resin CLEM of the Golgi apparatus and the endoplasmic reticulum in thin sections of the cells in Epon-embedded samples. To our knowledge, this is the first reported use of a far-red fluorescent protein retains its fluorescence after osmium staining and Epon-embedding, and it represents the first achievement of in-resin CLEM of both the Golgi apparatus and the endoplasmic reticulum in Epon-embedded samples.
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