ArticleCommunications biology2020
Correlation of fluorescence microscopy, electron microscopy, and NanoSIMS stable isotope imaging on a single tissue section.
Article in Communications biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 46 citations in OpenAlex.
- An emerging human eye disease is associated with aquatic virus zoonotic infection.Nature microbiology · 2026Article
- Computational Resolution Enhancement of Mitochondria monitoring in Multiple Organs using Intravital Two-Photon Microscopy.International journal of medical sciences · 2026Article
- Physiology and metabolism of eukaryotic microalgae involved in aquatic photosymbioses.The New phytologist · 2025Review
- A multimodal imaging pipeline to decipher cell-specific metabolic functions and tissue microenvironment dynamics.Nature protocols · 2025Review
- Non-invasive investigation of the morphology and optical properties of the upside-down jellyfishProceedings. Biological sciences · 2023Article
- Correlated cryo-SEM and CryoNanoSIMS imaging of biological tissue.BMC biology · 2023Article
- Correlative Light, Electron Microscopy and Raman Spectroscopy Workflow To Detect and Observe Microplastic Interactions with Whole Jellyfish.Environmental science & technology · 2023Article
- Correlative SIP-FISH-Raman-SEM-NanoSIMS links identity, morphology, biochemistry, and physiology of environmental microbes.ISME communications · 2022Article
- Quantitative correlative microscopy reveals the ultrastructural distribution of endogenous endosomal proteins.The Journal of cell biology · 2022Article
- Planktonic Aggregates as Hotspots for Heterotrophic Diazotrophy: The Plot Thickens.Frontiers in microbiology · 2022Review
- Different functional traits among closely related algal symbionts dictate stress endurance for vital Indo-Pacific reef-building corals.Global change biology · 2021Article
- Calculation and Interpretation of Substrate Assimilation Rates in Microbial Cells Based on Isotopic Composition Data Obtained by nanoSIMS.Frontiers in microbiology · 2021Article
- Amoebocytes facilitate efficient carbon and nitrogen assimilation in theProceedings. Biological sciences · 2020Article
- Heterotrophic Foraminifera Capable of Inorganic Nitrogen Assimilation.Frontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Correlative light and electron microscopy allows localization of specific molecules at the ultrastructural level in biological tissue but does not provide information about metabolic turnover or the distribution of labile molecules, such as micronutrients. We present a method to directly correlate (immuno)fluorescent microscopy, (immuno)TEM imaging and NanoSIMS isotopic mapping of the same tissue section, with nanometer-scale spatial precision. The process involves chemical fixation of the tissue, cryo sectioning, thawing, and air-drying under a thin film of polyvinyl alcohol. It permits to effectively retain labile compounds and strongly increases NanoSIMS sensitivity for
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