ArticleBMC complementary medicine and therapies2020
Phytochemical analysis and in vitro anti-proliferative activity of Viscum album ethanolic extracts.
Article in BMC complementary medicine and therapies, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
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- The cardioprotective potential of selected species of mistletoe.Frontiers in pharmacology · 2024Review
- Self-Nanoemulsifying Drug Delivery System (SNEDDS) Using Lipophilic Extract ofInternational journal of nanomedicine · 2024Article
- Mistletoe Extracts from Different Host Trees Disparately Inhibit Bladder Cancer Cell Growth and Proliferation.Cancers · 2023Article
- A Scoping Review of GenusPlants (Basel, Switzerland) · 2023Article
- The Anti-Inflammatory Activity ofPlants (Basel, Switzerland) · 2023Review
- Investigations on the morpho-anatomy and histochemistry of the European mistletoe: Viscum album L. subsp. album.Scientific reports · 2023Article
- PluronicPharmaceutics · 2022Article
- Phytochemical Profile and Antioxidant Capacity ofPlants (Basel, Switzerland) · 2022Article
- Biotechnological Potential of Different Organs of Mistletoe (Plants (Basel, Switzerland) · 2022Article
- RomanianPlants (Basel, Switzerland) · 2022Article
- Viscotoxin and lectin content in foliage and fruit of Viscum album L. on the main host trees of Hyrcanian forests.Scientific reports · 2022Article
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- Thermoresponsive Hydrogel ContainingPharmaceutics · 2021Article
- Anti-dengue activity of super critical extract and isolated oleanolic acid of Leucas cephalotes using in vitro and in silico approach.BMC complementary medicine and therapies · 2021Article
- Metabolomics by UHPLC-Q-TOF Reveals Host Tree-Dependent Phytochemical Variation inPlants (Basel, Switzerland) · 2021Article
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Authors and funding
13 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundViscum album L. (Santalaceae), commonly known as mistletoe, is a hemiparasitic plant traditionally used in complementary cancer treatment. Its antitumor potential is mostly attributed to the presence of aqueous soluble metabolites; however, the use of ethanol as solvent also permits the extraction of pharmacological compounds with antitumor potential. The clinical efficacy of mistletoe therapy inspired the present work, which focuses on ethanolic extracts (V. album "mother tinctures", MT) prepared from different host trees.
methodsSamples from three European subspecies (album, austriacum, and abietis) were harvested, and five different V. album-MT strains were prepared. The following phytochemical analyses were performed: thin layer chromatography (TLC), high-performance liquid chromatography (HPLC) and liquid chromatography-high resolution mass spectrometry (LC-HRMS). The proliferation assay was performed with WST-1 after incubation of tumor (Yoshida and Molt-4) and fibroblast cell lines (NIH/3 T3) with different MT concentrations (0.5 to 0.05% v/v). The cell death mechanism was investigated by flow cytometry (FACS) using Annexin V-7AAD.
resultsChemical analyses of MT showed the presence of phenolic acids, flavonoids and lignans. The MT flavonoid and viscotoxin contents (mg/g fresh weight) were highest in Quercus robur (9.67 ± 0.85 mg/g) and Malus domestica (3.95 ± 0.58 mg/mg), respectively. The viscotoxin isoform proportions (% total) were also different among the VA subspecies with a higher content of A3 in V. album growing on Abies alba (60.57 ± 2.13). The phytochemical compounds as well as the viscotoxin contents are probably related to the antitumor effects of MT. The cell death mechanisms evaluated by colorimetric and FACS methodologies involved necrotic damage, which was host tree-, time- and dose- dependent, with different selectivity to tumor cells. Mother tincture from V. album ssp. abietis was the most effective at inducing in vitro cellular effects, even when incubated at the smallest concentration tested, probably because of the higher content of VT A3.
conclusionOur results indicate the promising antitumor potential of Viscum album ethanolic extracts and the importance of botanical and phytochemical characterization for in vitro anti-proliferative effects.
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