ArticleInternational journal of molecular sciences2020
Single Nucleotide Polymorphisms as Practical Molecular Tools to Support European Chestnut Agrobiodiversity Management.
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- DNA Markers Unlock Hidden Plant Biodiversity.International journal of molecular sciences · 2026Article
- The FORGENIUS Genomic Resources: New Genotyping Tools and Genomic Data for 23 Forest Tree Species and Their Genetic Conservation Units.Molecular ecology resources · 2026Article
- Genetic Diversity and Population Structure of Phyllosphere-Associated Xanthomonas euvesicatoria Bacteria in Physalis pubescens Based on BOX-PCR and ERIC-PCR in China.The plant pathology journal · 2025Article
- A new database of chestnut DNA fingerprints for genetic diversity assessment, precise varietal identification, and traceability.Database : the journal of biological databases and curation · 2025Article
- Needle and Branch Trait Variation Analysis and Associated SNP Loci Mining inInternational journal of molecular sciences · 2024Article
- Health-Promoting Effects, Phytochemical Constituents and Molecular Genetic Profile of the Purple Carrot 'Purple Sun' (Nutrients · 2024Article
- Use of retrotransposon based iPBS markers for determination of genetic relationship among some Chestnut Cultivars (Castanea sativa Mill.) in Türkiye.Molecular biology reports · 2023Article
- Pilot Study on the Geographical Mapping of Genetic Diversity among European Chestnut (Plants (Basel, Switzerland) · 2023Article
- Genetic Diversity and Population Structure of Chinese Chestnut (Plants (Basel, Switzerland) · 2022Article
- Advances in genomics for diversity studies and trait improvement in temperate fruit and nut crops under changing climatic scenarios.Frontiers in plant science · 2022Review
- Endophytic Fungi and Ecological Fitness of Chestnuts.Plants (Basel, Switzerland) · 2021Review
- Genetic Diversity Assessment and Cultivar Identification of Cucumber (Plants (Basel, Switzerland) · 2021Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
European chestnut orchards are multifunctional agroforestry systems with a key role in environmental management. Their biodiversity is at risk of erosion and farmers do not have enough tools to protect and valorize traditional ecotypes. In particular, cost effective and reliable molecular markers for cultivar identification are lacking. The aim of this research was to develop a new molecular tool for varietal identification in European chestnuts. A set of cultivars was preliminarily characterized to evaluate the range of genetic diversity using random amplified polymorphic DNA (RAPD) markers. The genetic distances indicated a sufficiently wide variability range among tested genotypes and confirmed they were suitable for our goal. A single nucleotide polymorphism (SNP) mining within 64 expressed sequence tags (EST), covering all the linkage groups, was performed by high-resolution melting (HRM) and validated by target resequencing. Fifty-six SNPs were retrieved by monitoring the variability present on the whole set of considered cultivars in loci uniformly distributed on the genome. A subset of 37 SNPs was finally transformed into kompetitive allele-specific PCR (KASP) markers that were successfully evaluated for varietal discrimination. Three assays (C1083, G0115 and A5096) were identified as necessary and sufficient for distinguishing among the tested cultivars. The developed tools can be effectively exploited by stakeholders for improving the management of the European chestnut genetic resources.
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