Evidence map›Paper›PMID 32645991›Full record

ArticleMicromachines2020

Three-Dimensional Regeneration of Patient-Derived Intestinal Organoid Epithelium in a Physiodynamic Mucosal Interface-on-a-Chip.

Yong Cheol Shin, Woojung Shin, Domin Koh, Alexander Wu, Yoko M Ambrosini, Soyoun Min, S Gail Eckhardt, R Y Declan Fleming, Seung Kim, Sowon Park and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Micromachines, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.

0numbers the graph read from it
0cells of the map it votes in
49citing papers in PubMed
3.4field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

49 citing papers in PubMed, 87 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 2 countries.

Yong Cheol ShinDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USA.ORCID 0000-0002-1418-5929
Woojung ShinDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USA.ORCID 0000-0002-1780-0317
Domin KohDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USA.
Alexander WuDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USA.ORCID 0000-0001-5106-132X
Yoko M AmbrosiniDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USA.ORCID 0000-0002-9543-2660
Soyoun MinDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USA.
S Gail EckhardtDepartment of Oncology, Dell Medical School, The University of Texas at Austin, Austin, TX 78712, USA.
R Y Declan FlemingDepartment of Oncology, Dell Medical School, The University of Texas at Austin, Austin, TX 78712, USA.ORCID 0000-0003-1038-053X
Seung KimSeverance Fecal Microbiota Transplantation Center, Severance Hospital, Department of Pediatrics, Yonsei University College of Medicine, Seoul 03722, Korea.ORCID 0000-0003-4373-9828
Sowon ParkSeverance Fecal Microbiota Transplantation Center, Severance Hospital, Department of Pediatrics, Yonsei University College of Medicine, Seoul 03722, Korea.
Hong KohSeverance Fecal Microbiota Transplantation Center, Severance Hospital, Department of Pediatrics, Yonsei University College of Medicine, Seoul 03722, Korea.
Tae Kyung YooDepartment of Computer Art, College of Art and Technology, Chung-Ang University, Seoul 06974, Korea.
Hyun Jung KimDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USA.ORCID 0000-0001-5342-2870
The University of Texas at Austin · USYonsei University · KRChung-Ang University · KR

Funding

A personalized colorectal cancer-on-a-chip for assessing tumor-microbiome crosstalkR21CA236690 · NCI · UNIVERSITY OF TEXAS AT AUSTIN · PI KIM, HYUN JUNG · 2019 to 2021
$523k
Investigation of the Carcinogenic Effects of Bactericidal Antibiotics in the GutK00CA245801 · NCI · HARVARD UNIVERSITY · PI SHIN, WOOJUNG · 2021 to 2023
$278k
Deciphering the Role of Gut Microbiome in Colitis-Associated Colorectal Cancer Using a Patient-Specific Disease-on-a-ChipF99CA245801 · NCI · UNIVERSITY OF TEXAS AT AUSTIN · PI SHIN, WOOJUNG · 2019 to 2020
$75k
American College of Veterinary Internal Medicine Advance Research Fellowships 771066Burroughs Wellcome Fund Collaborative Research Travel Grant BWF 1019990.01Cancer Prevention and Research Institute of Texas RR160093Cancer Research Institute UTA18-000889Leona M. and Harry B. Helmsley Charitable Trust 1912-03604Ministry of Science and ICT, South Korea 2018M3A9H3025030National Cancer Institute of the National Institutes of Health R21CA236690National Cancer Institute of the National Institutes of Health (NIH/NCI) F99CA245801NCI NIH HHS F99 CA245801NCI NIH HHS K00 CA245801NCI NIH HHS R21 CA236690
6 · The paper itself

Abstract

The regeneration of the mucosal interface of the human intestine is critical in the host-gut microbiome crosstalk associated with gastrointestinal diseases. The biopsy-derived intestinal organoids provide genetic information of patients with physiological cytodifferentiation. However, the enclosed lumen and static culture condition substantially limit the utility of patient-derived organoids for microbiome-associated disease modeling. Here, we report a patient-specific three-dimensional (3D) physiodynamic mucosal interface-on-a-chip (PMI Chip) that provides a microphysiological intestinal milieu under defined biomechanics. The real-time imaging and computational simulation of the PMI Chip verified the recapitulation of non-linear luminal and microvascular flow that simulates the hydrodynamics in a living human gut. The multiaxial deformations in a convoluted microchannel not only induced dynamic cell strains but also enhanced particle mixing in the lumen microchannel. Under this physiodynamic condition, an organoid-derived epithelium obtained from the patients diagnosed with Crohn's disease, ulcerative colitis, or colorectal cancer independently formed 3D epithelial layers with disease-specific differentiations. Moreover, co-culture with the human fecal microbiome in an anoxic-oxic interface resulted in the formation of stochastic microcolonies without a loss of epithelial barrier function. We envision that the patient-specific PMI Chip that conveys genetic, epigenetic, and environmental factors of individual patients will potentially demonstrate the pathophysiological dynamics and complex host-microbiome crosstalk to target a patient-specific disease modeling.

Indexed as

co-culturedisease modelinggut-on-a-chipmicrobiomemucosal interfacemultiaxial deformationorganoidphysiodynamic

Identifiers

PMID32645991
PMCPMC7408321
OpenAlexW3038764318

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.