Evidence map›Paper›PMID 32645403›Full record

ArticleOsteoarthritis and cartilage2020

Human articular cartilage is orthotropic where microstructure, micromechanics, and chemistry vary with depth and split-line orientation.

K M Fischenich, J A Wahlquist, R L Wilmoth, L Cai, C P Neu, V L Ferguson

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Osteoarthritis and cartilage, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 27 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

K M FischenichDepartment of Mechanical Engineering, University of Colorado, Boulder, CO, USA.
J A WahlquistDepartment of Mechanical Engineering, University of Colorado, Boulder, CO, USA; Materials and Manufacturing Directorate, Air Force Research Lab, Wright-Patterson AFB, OH, USA.
R L WilmothDepartment of Mechanical Engineering, University of Colorado, Boulder, CO, USA.
L CaiWeldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA.
C P NeuDepartment of Mechanical Engineering, University of Colorado, Boulder, CO, USA; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA; BioFrontiers Institute, University of Colorado, Boulder, CO, USA; Biomedical Engineering Program, University of Colorado, Boulder, CO, USA.
V L FergusonDepartment of Mechanical Engineering, University of Colorado, Boulder, CO, USA; BioFrontiers Institute, University of Colorado, Boulder, CO, USA; Biomedical Engineering Program, University of Colorado, Boulder, CO, USA. Electronic address: virginia.ferguson@colorado.edu.
University of Colorado Boulder · USPurdue University West Lafayette · USUnited States Air Force Research Laboratory · US

Funding

Probing Osteoarthritis Pathogenesis by Noninvasive Imaging of Cartilage StrainR01AR063712 · NIAMS · UNIVERSITY OF COLORADO · PI NEU, COREY P · 2013 to 2023
$4.7M
Mechanically Stiff Hydrogels for Osteochondral Tissue EngineeringR01AR069060 · NIAMS · UNIVERSITY OF COLORADO · PI Stephanie J Bryant · 2016 to 2026
$4.1M
NIAMS NIH HHS R01 AR063712NIAMS NIH HHS R01 AR069060
6 · The paper itself

Abstract

objectiveQuantitative, micrometer length scale assessment of human articular cartilage is essential to enable progress toward new functional tissue engineering approaches, including utilization of emerging 3D bioprinting technologies, and for improved computational modeling of the osteochondral unit. Thus the objective of this study was to characterize the structural organization, material properties, and chemical composition of human skeletally mature articular cartilage with respect to depth and defined morphological features: normal to the articulating surface, parallel to the split-line, and transverse to the split-line.

methodThree samples from the lateral femoral condyles of 4 healthy adult donors (55-61 years old) were evaluated via histology, second harmonic generation, microindentation, and Raman spectroscopy. All metrics were evaluated as a function of depth and direction relative to the split-line.

resultsAll donors presented with intact and healthy tissue. Collagen fiber orientation varied significantly between testing directions and with increasing depth from the articular surface. Both compressive and tensile modulus increased significantly with depth and differed across the middle and deep zones and depended on orthogonal direction relative to the split-line. Similarly, matrix components varied with both depth and direction, where chondroitin sulfate steadily increased with depth while collagen prevalence was highest in the surface layer.

conclusionsMicroscale measurements of human articular cartilage demonstrate that properties are both depth-dependent and orthotropic and depend on the underlying tissue structure and composition. These findings improve upon existing knowledge establishing more accurate measurements, with greater degree of depth and spatial specificity, as inputs for tissue engineering and computational modeling.

Indexed as

Biomechanical PhenomenaCartilage, ArticularFemaleHumansMaleMiddle AgedSecond Harmonic Generation MicroscopySpectrum Analysis, RamanAnisotropyArticular cartilageIndentationRamanSHG

Identifiers

PMID32645403
PMCPMC7697147
OpenAlexW3039207072

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.