Evidence map›Paper›PMID 32645367›Full record

ArticleMolecular cell2020

A scoutRNA Is Required for Some Type V CRISPR-Cas Systems.

Lucas B Harrington, Enbo Ma, Janice S Chen, Isaac P Witte, Dov Gertz, David Paez-Espino, Basem Al-Shayeb, Nikos C Kyrpides, David Burstein, Jillian F Banfield and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed, 2 pooled it
4.1field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 2 syntheses or guidelines pooled it, 74 citations in OpenAlex.

  1. Engineering CRISPR guide RNAs for programmable RNA sensors.Biochemical Society transactions · 2023
    Pooled it
  2. Pooled it
  3. Molecular mechanisms and biotechnology applications of CRISPR-Cas12a.Nature reviews. Molecular cell biology · 2026
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 2 countries.

Lucas B HarringtonDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Enbo MaDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Janice S ChenDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Isaac P WitteDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Dov GertzSchool of Molecular Cell Biology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 6997801, Israel.
David Paez-EspinoJoint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Basem Al-ShayebDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Nikos C KyrpidesJoint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
David BursteinDepartment of Earth and Planetary Science, University of California, Berkeley, Berkeley, CA 94720, USA; School of Molecular Cell Biology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 6997801, Israel.
Jillian F BanfieldDepartment of Earth and Planetary Science, University of California, Berkeley, Berkeley, CA 94720, USA; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
Jennifer A DoudnaDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Molecular Biophysics & Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Gladstone Institutes, University of California, San Francisco, San Francisco, CA 94114, USA. Electronic address: doudna@berkeley.edu.
University of California, Berkeley · USLawrence Berkeley National Laboratory · USPlanetary Science Institute · USQB3 · USTel Aviv University · IL

Funding

Center for Genomic Editing and Recording: Development and Application of Next-Generation Genome and Epigenome Editing Methods to Advance the Study and Treatment of Human DiseaseRM1HG009490 · NHGRI · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · PI Brittany S. Adamson, Martin Joseph Ankrah Aryee · 2017 to 2026
$22.7M
Population Genomic Analysis of Gut Microbial Colonization in Premature InfantsR01AI092531 · NIAID · UNIVERSITY OF CALIFORNIA BERKELEY · PI Jillian Banfield, Michael Morowitz · 2011 to 2026
$11.3M
Expanding CRISPR-Cas editing technology through exploration of novel Cas proteins and DNA repair systemsU01AI142817 · NIAID · UNIVERSITY OF CALIFORNIA BERKELEY · PI BANFIELD, JILLIAN, DOUDNA, JENNIFER A · 2018 to 2022
$2.0M
Howard Hughes Medical InstituteNational Science Foundation 1817593NHGRI NIH HHS RM1 HG009490NIAID NIH HHS R01 AI092531NIAID NIH HHS U01 AI142817NIAID NIH HHS U01AI142817-02
6 · The paper itself

Abstract

CRISPR-Cas12c/d proteins share limited homology with Cas12a and Cas9 bacterial CRISPR RNA (crRNA)-guided nucleases used widely for genome editing and DNA detection. However, Cas12c (C2c3)- and Cas12d (CasY)-catalyzed DNA cleavage and genome editing activities have not been directly observed. We show here that a short-complementarity untranslated RNA (scoutRNA), together with crRNA, is required for Cas12d-catalyzed DNA cutting. The scoutRNA differs in secondary structure from previously described tracrRNAs used by CRISPR-Cas9 and some Cas12 enzymes, and in Cas12d-containing systems, scoutRNA includes a conserved five-nucleotide sequence that is essential for activity. In addition to supporting crRNA-directed DNA recognition, biochemical and cell-based experiments establish scoutRNA as an essential cofactor for Cas12c-catalyzed pre-crRNA maturation. These results define scoutRNA as a third type of transcript encoded by a subset of CRISPR-Cas genomic loci and explain how Cas12c/d systems avoid requirements for host factors including ribonuclease III for bacterial RNA-mediated adaptive immunity.

Indexed as

CRISPR-Cas SystemsBacteriaBacterial ProteinsBase SequenceClustered Regularly Interspaced Short Palindromic RepeatsDNA, BacterialEndodeoxyribonucleasesEscherichia coliGenome, BacterialNucleic Acid ConformationPhylogenyRNA, BacterialRNA, Guide, CRISPR-Cas SystemsRNA, Small UntranslatedSequence AlignmentSequence Homology, Nucleic AcidBacterial ProteinsDNA, BacterialEndodeoxyribonucleasesRNA, BacterialRNA, Guide, CRISPR-Cas SystemsRNA, Small UntranslatedCandidate Phyla Radiation (CPR) bacteriaCas12c (C2c3)Cas12d (CasY)CRISPR-cascrRNARuvC nuclease domainscoutRNAtracrRNA

Identifiers

PMID32645367
PMCPMC8196889
OpenAlexW3040712007

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.