ArticlePlant biotechnology journal2021
Nonaqueous fractionation and overexpression of fluorescent-tagged enzymes reveals the subcellular sites of L-theanine biosynthesis in tea.
Article in Plant biotechnology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 70 citations in OpenAlex.
- Metabolomics-Based Analysis of Dayezhong Fresh Tea Leaves: Effects of Cultivar and Tenderness on Black Tea Quality.Foods (Basel, Switzerland) · 2026Article
- The dual fate of L-theanine in tea: Metabolic regulation and processing transformations.Journal of advanced research · 2026Review
- CsMYB213-CsbHLH35 module regulates theanine biosynthesis in tea plants (Camellia sinensis L.).Plant biotechnology journal · 2025Article
- Characterization of nitrate use efficiency in tea plant (Horticulture research · 2025Article
- L-Theanine Metabolism in Tea Plants: Biological Functions and Stress Tolerance Mechanisms.Plants (Basel, Switzerland) · 2025Review
- Article
- Dissection of the spatial dynamics of biosynthesis, transport, and turnover of major amino acids in tea plants (Horticulture research · 2024Article
- Depicting the genetic and metabolic panorama of chemical diversity in the tea plant.Plant biotechnology journal · 2024Article
- Comprehensive dissection of variation and accumulation of free amino acids in tea accessions.Horticulture research · 2024Article
- Metabolome and Microbiome Analysis to Study the Flavor of Summer Black Tea Improved by Stuck Fermentation.Foods (Basel, Switzerland) · 2023Article
- L-Theanine Improves Locomotor Function in a Model of Multiple Sclerosis Mice.Archives of Razi Institute · 2023Article
- Article
- CsGDH2.1 negatively regulates theanine accumulation in late-spring tea plants (Horticulture research · 2023Article
- Nitrogen transport and assimilation in tea plant (Frontiers in plant science · 2023Review
- Mechanism underlying the carotenoid accumulation in shaded tea leaves.Food chemistry: X · 2022Article
- HPLC and high-throughput sequencing revealed higher tea-leaves quality, soil fertility and microbial community diversity in ancient tea plantations: compared with modern tea plantations.BMC plant biology · 2022Article
- Quantitative trait loci mapping for free amino acid content using an albino population and SNP markers provides insight into the genetic improvement of tea plants.Horticulture research · 2022Article
- Carbon and Nitrogen Metabolism Are Jointly Regulated During Shading in Roots and Leaves ofFrontiers in plant science · 2022Article
- Production of l-Theanine by Escherichia coli in the Absence of Supplemental Ethylamine.Applied and environmental microbiology · 2021Article
- Effects of Long-Term Nitrogen Fertilization on the Formation of Metabolites Related to Tea Quality in Subtropical China.Metabolites · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
l-Theanine is a specialized metabolite in the tea (Camellia sinensis) plant which can constitute over 50% of the total amino acids. This makes an important contribution to tea functionality and quality, but the subcellular location and mechanism of biosynthesis of l-theanine are unclear. Here, we identified five distinct genes potentially capable of synthesizing l-theanine in tea. Using a nonaqueous fractionation method, we determined the subcellular distribution of l-theanine in tea shoots and roots and used transient expression in Nicotiana or Arabidopsis to investigate in vivo functions of l-theanine synthetase and also to determine the subcellular localization of fluorescent-tagged proteins by confocal laser scanning microscopy. In tea root tissue, the cytosol was the main site of l-theanine biosynthesis, and cytosol-located CsTSI was the key l-theanine synthase. In tea shoot tissue, l-theanine biosynthesis occurred mainly in the cytosol and chloroplasts and CsGS1.1 and CsGS2 were most likely the key l-theanine synthases. In addition, l-theanine content and distribution were affected by light in leaf tissue. These results enhance our knowledge of biochemistry and molecular biology of the biosynthesis of functional tea compounds.
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