Evidence map›Paper›PMID 32643116›Full record

ArticlePathology oncology research : POR2020

Increased miR-20b Level in High Grade Cervical Intraepithelial Neoplasia.

Tímea Szekerczés, Ádám Galamb, Norbert Varga, Márta Benczik, Adrienn Kocsis, Krisztina Schlachter, András Kiss, Nándor Ács, Zsuzsa Schaff, Csaba Jeney and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pathology oncology research : POR, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
0.4field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it, 10 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Review
  5. Article
  6. Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 3 countries.

Tímea Szekerczés2nd Department of Pathology, Semmelweis University, Budapest, Hungary.ORCID http://orcid.org/0000-0002-7373-8730
Ádám GalambDepartment of Obstetrics and Gynecology, Semmelweis University, Üllői 78/A, Budapest, 1082, Hungary.
Norbert VargaNuffield Department of Clinical Neurosciences, Sleep & Circadian Neuroscience Institute, Oxford University, Oxford, UK.
Márta BenczikSYNLAB Hungary Kft, Budapest, Hungary.
Adrienn KocsisNEUMANN Diagnostics Ltd, Budapest, Hungary.
Krisztina Schlachter2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
András Kiss2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
Nándor ÁcsDepartment of Obstetrics and Gynecology, Semmelweis University, Üllői 78/A, Budapest, 1082, Hungary.
Zsuzsa Schaff2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
Csaba JeneyDepartment of Microsystems Engineering, Albert-Ludwigs University, Freiburg, Germany.
Gábor Lendvai2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
Gábor SobelDepartment of Obstetrics and Gynecology, Semmelweis University, Üllői 78/A, Budapest, 1082, Hungary. sobelg@gmail.com.
Semmelweis University · HUJohn von Neumann University · HUUniversity of Freiburg · DEUniversity of Oxford · GB

Funding

Hungarian Ministry of National Development #KMR-12-A FP7-HEALTH-2012-INNOVATION-1-Grant Agreement Number 306037Hungarian National Research Foundation OTKA PD105019
6 · The paper itself

Abstract

Cervical cancer is a common malignant tumor worldwide ranking fourth in incidence and mortality among females, which was reduced significantly by cytology screening and human papilloma virus (HPV) DNA testing. The specificity of cytology is high; however, the sensitivity is low, in contrast to the HPV DNA testing. Despite the success of these measures, new biomarkers are still considered to aim increasing sensitivity and specificity of screening and diagnosis. Significant alterations in microRNA (miRNA) expression have been detected in several cancers with variable consistency. To investigate the stratification role of miRNAs between normal epithelium and cervical intraepithelial neoplasia (CIN2-3), we screened the expression of 667 miRNAs to identify significant markers (n = 10), out of them 9 miRNAs were applied in the study (miR-20b, -24, -26a, -29b, -99a, -100, -147, -212, -515-3p) along with RNU48 and U6 as the references. To benchmark the miRNAs, 22 paired (tumor-free and tumor tissue pairs) laser microdissection-obtained cervical formalin fixed, paraffin embedded tissue samples were assayed. The expression of miR-20b was 2.4 times higher in CIN2-3 samples as compared to normal tissues (p < 0.0001). In the HPV16-positive subsets of the samples (n = 13), miR-20b showed 2.9-times elevation (p < 0.001), whereas miR-515 was 1.15-times downregulated (p < 0.05) in CIN2-3 as compared to normal tissue. These results suggest the potential value of miR-20b as a statification biomarker in order to differentiate neoplastic and non-tumorous cases.

Indexed as

AdultBiomarkers, TumorFemaleFollow-Up StudiesHumansMicroRNAsMiddle AgedPapillomaviridaePapillomavirus InfectionsPrognosisROC CurveUterine Cervical DysplasiaUterine Cervical NeoplasmsYoung AdultBiomarkers, TumorMicroRNAsMIRN20b microRNA, humanCervical cancerCervical intraepithelial neoplasia (CIN)Human papilloma virusmicroRNA

Identifiers

PMID32643116
PMCPMC7471155
OpenAlexW3042176879

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.