ArticlePlanta2020
Improving the genome editing efficiency of CRISPR/Cas9 in Arabidopsis and Medicago truncatula.
Article in Planta, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- The regulatory frameworks surrounding CRISPR-edited papaya and their impact on international commerce.Journal of the science of food and agriculture · 2026Article
- Genetic transformation and CRISPR/Cas12a-mediated gene editing of European beech (Fagus sylvatica L.) employing a transient protoplast system.Communications biology · 2026Article
- Efficient in-vitro regeneration and transformation for CRISPR/Cas9-mediated genome editing of phytoene desaturase (PDS) gene in pea (Pisum sativum L.).Plant cell reports · 2026Article
- Nitrogen Management in Crop-Soil-Environment Systems: Pathways Toward Sustainable and Climate-Resilient Agriculture.International journal of molecular sciences · 2026Review
- Advances in gene editing for legume improvement: technologies, progress, and prospects.Frontiers in genome editing · 2026Review
- The Agrobacterium-mediated genetic transformation: a gateway for efficient CRISPR/Cas9 gene editing in leguminous.Transgenic research · 2025Review
- Auxin affects gene editing efficiency through regulating chromatin accessibility and plant regeneration process.Horticulture research · 2025Article
- CRISPR/Cas9-mediated editing of carotenoid biosynthesis genes alters carotenoid concentrations in kiwifruit.BMC plant biology · 2025Article
- Heterotrimeric G-protein subunits regulate plant architecture, pod development, seed size, and symbiotic nodulation inaBIOTECH · 2025Article
- Applying conventional and cell-type-specific CRISPR/Cas9 genome editing in legume plants.aBIOTECH · 2025Review
- Natural Selection as the Primary Driver of Codon Usage Bias in the Mitochondrial Genomes of ThreeGenes · 2025Article
- Harnessing promoter elements to enhance gene editing in plants: perspectives and advances.Plant biotechnology journal · 2025Review
- Genome editing for grass improvement and future agriculture.Horticulture research · 2025Article
- CRISPR/Cas9- and Cas3-mediated modification of copy number variation in rice.Frontiers in genome editing · 2025Article
- CRISPR-Based Editing of thePlants (Basel, Switzerland) · 2024Article
- Article
- Versatile Cloning Strategy for Efficient Multigene Editing inBio-protocol · 2024Article
- A dual-function selection system enables positive selection of multigene CRISPR mutants and negative selection ofaBIOTECH · 2024Article
- CRISPR/Cas9-Mediated Generation of Mutant Lines inBiology · 2024Article
- Crop genome editing through tissue-culture-independent transformation methods.Frontiers in genome editing · 2024Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionAn improved CRISPR/Cas9 system with the Arabidopsis UBQ10 promoter-driven Cas9 exhibits consistently high mutation efficiency in Arabidopsis and M. truncatula. CRISPR/Cas9 is a powerful genome editing technology that has been applied in several crop species for trait improvement due to its simplicity, versatility, and specificity. However, the mutation efficiency of CRISPR/Cas9 in Arabidopsis and M. truncatula (Mt) is still challenging and inconsistent. To analyze the functionality of the CRISPR/Cas9 system in two model dicot species, four different promoter-driven Cas9 systems to target phytoene desaturase (PDS) genes were designed. Agrobacterium-mediated transformation was used for the delivery of constructed vectors to host plants. Phenotypic and genotypic analyses revealed that the Arabidopsis UBQ10 promoter-driven Cas9 significantly improves the mutation efficiency to 95% in Arabidopsis and 70% in M. truncatula. Moreover, the UBQ10-Cas9 system yielded 11% homozygous mutants in the T1 generation in Arabidopsis. Sequencing analyses of mutation events indicated that single-nucleotide insertions are the most frequent events in Arabidopsis, whereas multi-nucleotide deletions are dominant in bi-allelic and mono-allelic homozygous mutants in M. truncatula. Taken together, the UBQ10 promoter facilitates the best improvement in the CRISPR/Cas9 efficiency in PDS gene editing, followed by the EC1.2 promoter. Consistently, the improved UBQ10-Cas9 vector highly enhanced the mutation efficiency by four-fold over the commonly used 35S promoter in both dicot species.
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