Evidence map›Paper›PMID 32641717›Full record

ArticleScientific reports2020

Near-chromosome level genome assembly of the fruit pest Drosophila suzukii using long-read sequencing.

Mathilde Paris, Roxane Boyer, Rita Jaenichen, Jochen Wolf, Marianthi Karageorgi, Jack Green, Mathilde Cagnon, Hugues Parinello, Arnaud Estoup, Mathieu Gautier and 2 more

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
8.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 63 citations in OpenAlex.

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  20. Evaluation of AdditionalFrontiers in bioengineering and biotechnology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 7 institutions in 4 countries.

Mathilde ParisAix-Marseille Université, CNRS, IBDM, Institut de Biologie du Développement de Marseille, Marseille Cedex 9, France.
Roxane BoyerAix-Marseille Université, CNRS, IBDM, Institut de Biologie du Développement de Marseille, Marseille Cedex 9, France.
Rita JaenichenLudwig-Maximilians Universität München, Fakultät für Biologie, Biozentrum, Grosshaderner Strasse 2, 82152, Planegg-Martinsried, Germany.
Jochen WolfLudwig-Maximilians Universität München, Fakultät für Biologie, Biozentrum, Grosshaderner Strasse 2, 82152, Planegg-Martinsried, Germany.
Marianthi KarageorgiAix-Marseille Université, CNRS, IBDM, Institut de Biologie du Développement de Marseille, Marseille Cedex 9, France.
Jack GreenAix-Marseille Université, CNRS, IBDM, Institut de Biologie du Développement de Marseille, Marseille Cedex 9, France.
Mathilde CagnonLudwig-Maximilians Universität München, Fakultät für Biologie, Biozentrum, Grosshaderner Strasse 2, 82152, Planegg-Martinsried, Germany.
Hugues ParinelloMGX, MGX, Biocampus Montpellier, CNRS, INSERM, University of Montpellier, Montpellier, France.
Arnaud EstoupCBGP, INRA, CIRAD, IRD, Montpellier SupAgro, University of Montpellier, Montpellier, France.
Mathieu GautierCBGP, INRA, CIRAD, IRD, Montpellier SupAgro, University of Montpellier, Montpellier, France. mathieu.gautier@inrae.fr.
Nicolas GompelLudwig-Maximilians Universität München, Fakultät für Biologie, Biozentrum, Grosshaderner Strasse 2, 82152, Planegg-Martinsried, Germany. gompel@bio.lmu.de.
Benjamin Prud'hommeAix-Marseille Université, CNRS, IBDM, Institut de Biologie du Développement de Marseille, Marseille Cedex 9, France. benjamin.prudhomme@univ-amu.fr.
Ludwig-Maximilians-Universität München · DECentre de Biologie et de Gestion des Populations · FRInstitut de Biologie du Développement Marseille · FRAix-Marseille Université · FRCentre National de la Recherche Scientifique · FRInserm · FRUniversity of California, Berkeley · US

Funding

European Research Council 615789
6 · The paper itself

Abstract

Over the past decade, the spotted wing Drosophila, Drosophila suzukii, has invaded Europe and America and has become a major agricultural pest in these areas, thereby prompting intense research activities to better understand its biology. Two draft genome assemblies already exist for this species but contain pervasive assembly errors and are highly fragmented, which limits their values. Our purpose here was to improve the assembly of the D. suzukii genome and to annotate it in a way that facilitates comparisons with D. melanogaster. For this, we generated PacBio long-read sequencing data and assembled a novel, high-quality D. suzukii genome assembly. It is one of the largest Drosophila genomes, notably because of the expansion of its repeatome. We found that despite 16 rounds of full-sib crossings the D. suzukii strain that we sequenced has maintained high levels of polymorphism in some regions of its genome. As a consequence, the quality of the assembly of these regions was reduced. We explored possible origins of this high residual diversity, including the presence of structural variants and a possible heterogeneous admixture pattern of North American and Asian ancestry. Overall, our assembly and annotation constitute a high-quality genomic resource that can be used for both high-throughput sequencing approaches, as well as manipulative genetic technologies to study D. suzukii.

Indexed as

Contig MappingAnimalsChromosomes, InsectDrosophilaFemaleFruitGenome, InsectGenomicsHigh-Throughput Nucleotide SequencingMalePolymorphism, GeneticWhole Genome Sequencing

Identifiers

PMID32641717
PMCPMC7343843
OpenAlexW3041433010

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.