Novel Proteome Extraction Method Illustrates a Conserved Immunological Signature of MSI-H Colorectal Tumors.

Elez D Vainer, Juliane Kania-Almog, Ghadeer Zatara, Yishai Levin, Gilad W Vainer

Open access · hybridAbstract read

In one paragraph

Article in Molecular & cellular proteomics : MCP, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact, top 91% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

SpectiCal:Journal of proteome research · 2024
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 1 country.

Elez D VainerDepartment of Gastroenterology, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.

Juliane Kania-AlmogTel Aviv Sourasky Medical Center, Sackler School of Medicine, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel.

Ghadeer ZataraDepartment of Pathology, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.

Yishai LevinDe Botton Institute for Protein Profiling, The Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel.

Gilad W VainerDepartment of Pathology, Hadassah-Hebrew University Medical Center, Jerusalem, Israel. Electronic address: giladwv@gmail.com.

Hadassah Medical Center · ILHebrew University of Jerusalem · ILTel Aviv Sourasky Medical Center · ILWeizmann Institute of Science · IL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Using a simple, environment friendly proteome extraction (TOP), we were able to optimize the analysis of clinical samples. Using our TOP method we analyzed a clinical cohort of microsatellite stable (MSS) and unstable (MSI-H) colorectal carcinoma (CRC). We identified a tumor cell specific, STAT1-centered, immune signature expressed by the MSI-H tumor cells. We then showed that long, but not short, exposure to Interferon-γ induces a similar signature

Indexed as

Microsatellite InstabilityCluster AnalysisColorectal NeoplasmsFormaldehydeHumansInterferon-gammaParaffin EmbeddingProteomeProteomicsSTAT1 Transcription FactorSTAT3 Transcription FactorTissue FixationFormaldehydeInterferon-gammaProteomeSTAT1 Transcription FactorSTAT3 Transcription FactorClinical proteomicscolorectal cancerimmunohistochemistryimmunologyinflammatory responselabel-free quantificationmolecular biology

Identifiers

PMID32641473

DOI10.1074/mcp.RA120.002152

PMCPMC8015011

OpenAlexW3041377547

What OpenQuestion holds

Textmetadata

LicenceCC BY

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.