Evidence map›Paper›PMID 32640616›Full record

ArticleAnimals : an open access journal from MDPI2020

Validation of Real-time PCR Reference Genes of Muscle Metabolism in Harvested Spiny-Cheek Crayfish (

Natalia Śmietana, Remigiusz Panicz, Małgorzata Sobczak, Piotr Eljasik, Przemysław Śmietana

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animals : an open access journal from MDPI, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Natalia ŚmietanaDepartment of Meat Sciences, Faculty of Food Sciences and Fisheries, West Pomeranian University of Technology in Szczecin, 4 Kazimierza Królewicza Street, 71-550 Szczecin, Poland.
Remigiusz PaniczDepartment of Meat Sciences, Faculty of Food Sciences and Fisheries, West Pomeranian University of Technology in Szczecin, 4 Kazimierza Królewicza Street, 71-550 Szczecin, Poland.ORCID 0000-0003-4858-2233
Małgorzata SobczakDepartment of Meat Sciences, Faculty of Food Sciences and Fisheries, West Pomeranian University of Technology in Szczecin, 4 Kazimierza Królewicza Street, 71-550 Szczecin, Poland.
Piotr EljasikDepartment of Meat Sciences, Faculty of Food Sciences and Fisheries, West Pomeranian University of Technology in Szczecin, 4 Kazimierza Królewicza Street, 71-550 Szczecin, Poland.ORCID 0000-0001-7220-5484
Przemysław ŚmietanaUniversity of Szczecin, Institute of Marine and Environmental Sciences, 18 Adama Mickiewicza Street, 70-383 Szczecin, Poland.
West Pomeranian University of Technology in Szczecin · PLUniversity of Szczecin · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Real-time quantitative reverse transcription PCR (RT-qPCR) is a sensitive and broadly used technique of assessing gene activity. To obtain a reliable result, stably expressed reference genes are essential for normalization of transcripts in various samples. To our knowledge, this is the first systematic analysis of reference genes for normalization of RT-qPCR data in spiny-cheek crayfish (

Indexed as

abdomen musclesendogenous control genesferritinfreshwater crayfishmoltingtroponin c

Identifiers

PMID32640616
PMCPMC7401605
OpenAlexW3040673479

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.