ArticleAnalytical chemistry2020
Increasing the Coverage of a Mass Spectral Library of Milk Oligosaccharides Using a Hybrid-Search-Based Bootstrapping Method and Milks from a Wide Variety of Mammals.
Article in Analytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Analysis of the Milk Oligosaccharides Spectrum and Sialylation Status of Milk from West African Dwarf Goat and East Friesian Sheep.ACS omega · 2026Article
- Seal milk oligosaccharides rival human milk complexity and exhibit functional dynamics during lactation.Nature communications · 2025Article
- Robust and High-Resolution All-Ion Fragmentation LC-ESI-IM-MS Analysis for In-Depth Characterization or Profiling of Up to 200 Human Milk Oligosaccharides.Analytical chemistry · 2025Article
- Unusual free trisaccharides in caprine colostrum discovered by logically derived sequence tandem mass spectrometry.Scientific reports · 2025Article
- MilkOligoCorpus: A semantically annotated resource for knowledge extraction on mammalian milk oligosaccharides.PloS one · 2025Article
- MilkOligoThesaurus, a dataset of mammalian milk oligosaccharide synonyms.Data in brief · 2024Article
- Recent Advances in the Microbial Production of Human Milk Oligosaccharides.Current opinion in food science · 2024Article
- Safety of lacto-N-fucopentaose I/2'-fucosyllactose (LNFP-I/2'-FL) mixture as a novel food pursuant to Regulation (EU) 2015/2283.EFSA journal. European Food Safety Authority · 2023Article
- Creation of a milk oligosaccharide database, MilkOligoDB, reveals common structural motifs and extensive diversity across mammals.Scientific reports · 2023Article
- Piperitone (Biomolecules · 2023Article
- The critical role that spectral libraries play in capturing the metabolomics community knowledge.Metabolomics : Official journal of the Metabolomic Society · 2022Review
- In silico analysis of the human milk oligosaccharide glycome reveals key enzymes of their biosynthesis.Scientific reports · 2022Article
- Unusual free oligosaccharides in human bovine and caprine milk.Scientific reports · 2022Article
- Identifying Mixtures of Isomeric Human Milk Oligosaccharides by the Decomposition of IR Spectral Fingerprints.Analytical chemistry · 2021Article
- Extraction and Quantitative Analysis of Goat Milk Oligosaccharides: Composition, Variation, Associations, and 2'-FL Variability.Journal of agricultural and food chemistry · 2021Article
- Longitudinal Profiles of Dietary and Microbial Metabolites in Formula- and Breastfed Infants.Frontiers in molecular biosciences · 2021Article
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9 authors.
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Abstract
This study significantly expands both the scope and method of identification for construction of a previously reported tandem mass spectral library of 74 human milk oligosaccharides (HMOs) derived from results of combined LC-MS/MS experiments and comprehensive structural analysis of HMOs. In the present work, a hybrid search "bootstrap" identification method was employed that substantially broadens the coverage of milk oligosaccharides and thereby increases utility use of a spectrum library-based method for the rapid tentative identification of all distinguishable glycans in milk. This involved hybrid searching of the previous library, which was itself constructed using the hybrid search of oligosaccharide spectra in the NIST 17 Tandem MS Library. The general approach appears applicable to library construction of other classes of compounds. The coverage of oligosaccharides was significantly extended using milks from a variety of mammals, including bovine, Asian buffalo, African lion, and goat. This new method led to the identification of another 145 oligosaccharides, including an additional 80 HMOs from reanalysis of human milk. The newly identified compounds were added to a freely available mass spectral reference database of 219 milk oligosaccharides. We also provide suggestions to overcome several limitations and pitfalls in the interpretation of spectra of unknown oligosaccharides.
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