ArticleBiotechnology letters2020
Enhancement of protoplast preparation and regeneration of Hirsutella sinensis based on process optimization.
Article in Biotechnology letters, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 18 citations in OpenAlex.
- Optimization of protoplast based DNA isolation and genome analysis in a gamma-irradiated Aspergillus niger mutant strain.Journal of applied genetics · 2026Article
- Process optimization for protoplast preparation from Grifola frondosa and development of a high-yield mutant with enhanced mycelial growth and protein production.BMC biotechnology · 2026Article
- An Efficient and Stable PEG-Mediated Transformation System for Medicinal FungusJournal of fungi (Basel, Switzerland) · 2026Article
- A PEG-mediated protoplast transformation system for EGFP labeling of the shot-hole pathogen Wilsonomyces carpophilus.Frontiers in microbiology · 2026Article
- Enhanced bafilomycin A1 production in Streptomyces Griseobrunneus via protoplast fusion combined with high-throughput screening.World journal of microbiology & biotechnology · 2025Article
- Establishment of Transient Transformation Systems in Welsh Onion (Plants (Basel, Switzerland) · 2025Article
- PEG-Mediated Protoplast Transformation ofJournal of fungi (Basel, Switzerland) · 2025Article
- Optimization of Protoplast Preparation and Establishment of PEG-Mediated Genetic Transformation Method inJournal of fungi (Basel, Switzerland) · 2025Article
- Improving Geldanamycin Production inMicroorganisms · 2025Article
- Optimization of Protoplast Preparation Conditions inJournal of fungi (Basel, Switzerland) · 2024Article
- Establishment of anCurrent issues in molecular biology · 2024Article
- Development of lead (Pb) tolerant strain by protoplast technology and their remediation.World journal of microbiology & biotechnology · 2023Article
- CRISPR/Cas9 RNP-assisted validation of palmarumycin biosynthetic gene cluster inFrontiers in microbiology · 2022Article
- Optimization of Protoplast Preparation and Establishment of Genetic Transformation System of an Arctic-Derived FungusFrontiers in microbiology · 2022Article
- Improvement of cordycepin production by an isolated Paecilomyces hepiali mutant from combinatorial mutation breeding and medium screening.Bioprocess and biosystems engineering · 2021Article
- Genetic Manipulation of the Brassicaceae Smut FungusJournal of fungi (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
objectiveTo explore the optimal methods for the protoplast preparation and regeneration of Hirsutella sinensis by optimizing the limiting factors.
resultsDuring the treatment of enzymatic protoplast preparation, mycelium cultured for 7 days was the optimal start material. The maximum protoplast preparation rate of 4.3 × 10
conclusionsThe preparation and regeneration of H. sinensis protoplasts was firstly established based on process optimization and it provided a foundation for the study of H. sinensis mutagenesis.
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