Evidence map›Paper›PMID 32637856›Full record

ArticleAPL bioengineering2020

Stiffness based enrichment of leukemia cells using microfluidics.

Muhymin Islam, Abhishek Raj, Brynn McFarland, Hannah Maxine Brink, Jordan Ciciliano, Meredith Fay, David Richard Myers, Christopher Flowers, Edmund K Waller, Wilbur Lam and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in APL bioengineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Muhymin IslamGeorge W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 801 Ferst Drive, Atlanta, Georgia 30332-0405, USA.
Abhishek RajGeorge W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 801 Ferst Drive, Atlanta, Georgia 30332-0405, USA.
Brynn McFarlandThe School of Biological Sciences, Georgia Institute of Technology, 310 Ferst Drive, Atlanta, Georgia 30332-0535, USA.
Hannah Maxine BrinkThe School of Biological Sciences, Georgia Institute of Technology, 310 Ferst Drive, Atlanta, Georgia 30332-0535, USA.
Jordan CicilianoWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 313 Ferst Drive, Atlanta, Georgia 30332-0535, USA.
Meredith FayWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 313 Ferst Drive, Atlanta, Georgia 30332-0535, USA.
David Richard MyersWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 313 Ferst Drive, Atlanta, Georgia 30332-0535, USA.
Christopher FlowersWinship Cancer Institute, Emory School of Medicine, 1365 Clifton NE Rd.: Atlanta, Georgia 30322, USA.
Edmund K WallerWinship Cancer Institute, Emory School of Medicine, 1365 Clifton NE Rd.: Atlanta, Georgia 30322, USA.
Wilbur LamWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 313 Ferst Drive, Atlanta, Georgia 30332-0535, USA.
Alexander AlexeevGeorge W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 801 Ferst Drive, Atlanta, Georgia 30332-0405, USA.ORCID https://orcid.org/0000-0002-8285-0003
Todd SulchekORCID https://orcid.org/0000-0003-4196-6293
Georgia Institute of Technology · USEmory Healthcare · US

Funding

Label-free microfluidic enrichment of cancer cells from noncancer cells in ascites fluidR21CA191243 · NCI · GEORGIA INSTITUTE OF TECHNOLOGY · PI MCDONALD, JOHN F, SULCHEK, TODD · 2016 to 2019
$593k
Microfluidic Monitoring of Single Cell Elasticity, Viscoelasticity, and PlasticityR21EB020977 · NIBIB · GEORGIA INSTITUTE OF TECHNOLOGY · PI ALEXEEV, ALEXANDER, LAM, WILBUR A · 2015 to 2016
$405k
NCI NIH HHS R21 CA191243NIBIB NIH HHS R21 EB020977
6 · The paper itself

Abstract

To improve the survival rate of cancer patients, new diagnosis strategies are necessary to detect lower levels of cancer cells before and after treatment regimens. The scarcity of diseased cells, particularly in residual disease after treatment, demands highly sensitive detection approaches or the ability to enrich the diseased cells in relation to normal cells. We report a label-free microfluidic approach to enrich leukemia cells from healthy cells using inherent differences in cell biophysical properties. The microfluidic device consists of a channel with an array of diagonal ridges that recurrently compress and translate flowing cells in proportion to cell stiffness. Using devices optimized for acute T cell leukemia model Jurkat, the stiffer white blood cells were translated orthogonally to the channel length, while softer leukemia cells followed hydrodynamic flow. The device enriched Jurkat leukemia cells from white blood cells with an enrichment factor of over 760. The sensitivity, specificity, and accuracy of the device were found to be

Identifiers

PMID32637856
PMCPMC7332299
OpenAlexW3039553678

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.