ReviewSynthetic and systems biotechnology2020
Site directed mutagenesis as a precision tool to enable synthetic biology with engineered modular polyketide synthases.
Review in Synthetic and systems biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Enhanced Production of Astaxanthin and Zeaxanthin by Paracoccus Sp. LL1 Through Random Mutagenesis.Biotechnology and applied biochemistry · 2026Article
- Ancestral Sequence Reconstruction for Novel Bifunctional Glutathione Synthetase with Enhanced Thermostability and Catalytic Efficiency.Foods (Basel, Switzerland) · 2026Article
- Stabilization of Enzymes via Covalent Tethering to Appropriately Tuned Random Copolymer Brushes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Engineering Polyketide Stereocenters with Ketoreductase Domain Exchanges.Journal of the American Chemical Society · 2025Article
- Plug-and-play engineering of modular polyketide synthases.Nature chemical biology · 2025Article
- Linking a polyketide synthase gene cluster to 6-pentyl-alpha-pyrone, a Trichoderma metabolite with diverse bioactivities.Microbial cell factories · 2025Article
- Functional characterization of key polyketide synthases by integrated metabolome and transcriptome analysis on curcuminoid biosynthesis inSynthetic and systems biotechnology · 2022Article
- Design of Artificial Enzymes Bearing Several Active Centers: New Trends, Opportunities and Problems.International journal of molecular sciences · 2022Review
- Deciphering and engineering the polyunsaturated fatty acid synthase pathway from eukaryotic microorganisms.Frontiers in bioengineering and biotechnology · 2022Review
- Development of Genetically Encoded Biosensors for Reporting the Methyltransferase-Dependent Biosynthesis of Semisynthetic Macrolide Antibiotics.ACS synthetic biology · 2021Article
- Boosted activity by engineering the enzyme microenvironment in cascade reaction: A molecular understanding.Synthetic and systems biotechnology · 2021Article
- Natural products research in the modern age.Synthetic and systems biotechnology · 2020Article
Corrections and comments
- Erratum issued
- Erratum issued
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Modular polyketide synthases (PKSs) are a multidomain megasynthase class of biosynthetic enzymes that have great promise for the development of new compounds, from new pharmaceuticals to high value commodity and specialty chemicals. Their colinear biosynthetic logic has been viewed as a promising platform for synthetic biology for decades. Due to this colinearity, domain swapping has long been used as a strategy to introduce molecular diversity. However, domain swapping often fails because it perturbs critical protein-protein interactions within the PKS. With our increased level of structural elucidation of PKSs, using judicious targeted mutations of individual residues is a more precise way to introduce molecular diversity with less potential for global disruption of the protein architecture. Here we review examples of targeted point mutagenesis to one or a few residues harbored within the PKS that alter domain specificity or selectivity, affect protein stability and interdomain communication, and promote more complex catalytic reactivity.
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