Evidence map›Paper›PMID 32637484›Full record

ArticleData in brief2020

Data describing expression of formyl peptide receptor 2 in human articular chondrocytes.

Alexander Strid Holmertz, Charlotte A Jonsson, Maziar Mohaddes, Christina Lundqvist, Huamei Forsman, Inger Gjertsson, Karin Önnheim

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Data in brief, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Alexander Strid HolmertzDepartment of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, Gothenburg University, Göteborg, Sweden.
Charlotte A JonssonDepartment of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, Gothenburg University, Göteborg, Sweden.
Maziar MohaddesDepartment of Orthopaedics, Institute of Clinical Sciences, Sahlgrenska Academy, Gothenburg University, Göteborg, Sweden.
Christina LundqvistDepartment of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, Gothenburg University, Göteborg, Sweden.
Huamei ForsmanDepartment of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, Gothenburg University, Göteborg, Sweden.
Inger GjertssonDepartment of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, Gothenburg University, Göteborg, Sweden.
Karin ÖnnheimDepartment of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, Gothenburg University, Göteborg, Sweden.
University of Gothenburg · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The formyl peptide receptor 2 (FPR2) belongs to the family of seven-transmembrane G protein-coupled receptors (GPCR) and are expressed by many different cells but mainly studied in immune cells. FPR2 is involved in host defense against bacterial infections and clearance of damaged cells through the oxidative burst and chemotaxis of neutrophils. In addition, FPR2 has also been implicated as an immunomodulator in sterile inflammations, e.g. inflammatory joint diseases. Here we present data regarding FPR2 expression in human articular chondrocytes, isolated from healthy individuals and osteoarthritic patients, on both mRNA and protein level using qPCR and Imagestream flow cytometry. We also present data after receptor stimulation and monitoring of production of nitric oxide, reactive oxygen species, IL-6, IL-8 and MMP-3. The presented data show that human articular chondrocytes from patients with osteoarthritis as well as from healthy individuals express FPR2 both at mRNA and protein level. The biological relevance of FPR2 expression in chondrocytes needs to be further investigated.

Indexed as

Articular cartilageChondrocyteCL, ChemiluminescenceCT, Cycle thresholdDMEM, Dulbecco´s modified eagle mediumECM, Extra cellular matrixFACS, Fluorescence-activated cell sortingFBS, Fetal bovine serumFormyl peptide receptorFPR, Formyl peptide receptorGAPDH, Glyceraldehyde 3-phosphate dehydrogenaseGPCR, G protein-coupled receptorHI, Healthy individualHRP, Horse radish peroxidaseHumanIL-1β, Interleukin 1 betaKRG, Krebs Ringer phosphate bufferMMP, Matrix metalloproteinaseNO, Nitric oxideOA, OsteoarthritisOsteoarthritisPBMC, Peripheral blood mononuclear cellsqPCR, Quantitative polymerase chain reactionRLU, Relative light unitsROS, Reactive oxygen species

Identifiers

PMID32637484
PMCPMC7326719
OpenAlexW3034751943

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.