ArticleMethodsX2020
Method for improved integrity of RNA isolated from Matrigel cultures.
Article in MethodsX, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Transcriptomic Analyses of Normal Human Pancreata Reveal the Presence of Cancer Subtypes that Correlate with Acinar Ductal Metaplasia and Donor Ancestry.Cancer research communications · 2026Article
- Rethinking Matrigel: The Complex Journey to Matrix Alternatives in Organoid Culture.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Dimethyl Sulfoxide Conditions Induced Pluripotent Stem Cells for more Efficient Nephron Progenitor and Kidney Organoid Differentiation.Stem cell reviews and reports · 2025Article
- Article
- Small molecular weight epigenetic inhibitors modulate the extracellular matrix during pancreatic acinar ductal metaplasia.Biochemical and biophysical research communications · 2024Article
- Resistance of HNSCC cell models to pan-FGFR inhibition depends on the EMT phenotype associating with clinical outcome.Molecular cancer · 2024Article
- Epigenetic small-molecule screen for inhibition and reversal of acinar ductal metaplasia in mouse pancreatic organoids.Frontiers in pharmacology · 2024Article
- SELENOF Controls Proliferation and Cell Death in Breast-Derived Immortalized and Cancer Cells.Cancers · 2023Article
- Crypt-like patterned electrospun nanofibrous membrane and probiotics promote intestinal epithelium models close to tissues.Applied microbiology and biotechnology · 2023Article
- Evaluation of Apoptosis-related Genes and Hormone Secretion Profiles Using Three Dimensional Culture System of Human Testicular Organoids.Galen medical journal · 2023Article
- Transcriptional Profile of Human Pancreatic Acinar Ductal Metaplasia.Gastro hep advances · 2023Article
- Pharmacological inhibition and reversal of pancreatic acinar ductal metaplasia.Cell death discovery · 2022Article
- Mechanism of action and therapeutic route for a muscular dystrophy caused by a genetic defect in lipid metabolism.Nature communications · 2022Article
- Viable human brain microvessels for the study of aging and neurodegenerative diseases.Microvascular research · 2022Article
Corrections and comments
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Authors and funding
5 authors.
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Abstract
Matrigel is a commercially available substrate that is derived from the extracellular matrix. Matrigel is widely used in cell culture experiments such as the transdifferentiation of primary pancreatic acini to ductal epithelial-like cells. Difficulty arises during gene expression analysis for cells cultured on Matrigel because residual RNA in the Matrigel will not only contribute to the poor integrity of RNA isolated from Matrigel cultures, but also will impact the gene expression data. We report here a simple method of removing Matrigel from primary cultures of human or mouse pancreatic acini. Following the experiment, the cultures are placed on wet ice to liquefy the Matrigel. The cell and Matrigel mixture is then centrifuged at low speed to separate the pancreatic cells from the Matrigel solution that resides in the supernatant. RNA isolated from the pelleted cells has high integrity and may be readily used for gene expression analysis such as quantitative reverse transcription PCR.
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