ArticleBiomedical optics express2020
Procedure and protocols for optical imaging of cerebral blood flow and hemodynamics in awake mice.
Article in Biomedical optics express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 14 citations in OpenAlex.
- Preclinical in-vivo cerebrovascular imaging in rodent models: Methods and applications in Alzheimer's disease.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2026Review
- Variable Temporal Interval OCTA Velocimetry Enables Wide-Range Hemodynamic Mapping in Hyperoxia Models.Investigative ophthalmology & visual science · 2026Article
- Optical coherence tomography angiography reveals structural and hemodynamic remodeling of cerebral penetrating vessels owing to aging.Biomedical optics express · 2026Article
- Inducing and monitoring photothrombotic stroke in anesthetic neuroprotection-free mice using functional photoacoustic microscopy.Neurophotonics · 2026Article
- Magnetic resonance imaging cerebral blood perfusion measurement in awake mice.Journal of neuroscience methods · 2025Article
- Microimager: a flexible thin-film miniaturized endoscope for optical biomedical imaging.Biomedical optics express · 2025Article
- Non-invasive real-time pulsed Doppler assessment of blood flow in mouse ophthalmic artery.Cell reports methods · 2025Article
- Optical coherence tomography (OCT) and OCT angiography: Technological development and applications in brain science.Theranostics · 2025Review
- Wearable optical coherence tomography angiography probe with extended depth of field.Journal of biomedical optics · 2025Article
- Deep Imaging to Dissect Microvascular Contributions to White Matter Degeneration in Rodent Models of Dementia.Stroke · 2023Review
- Visualization of blood-brain barrier disruption with dual-wavelength high-resolution photoacoustic microscopy.Biomedical optics express · 2022Article
- Quantitative Hemodynamic Measurements in Cortical Vessels Using Functional Ultrasound Imaging.Frontiers in neuroscience · 2022Article
- Differences in cerebral blood vasculature and flow in awake and anesthetized mouse cortex revealed by quantitative optical coherence tomography angiography.Journal of neuroscience methods · 2021Article
- Location Matters: Navigating Regional Heterogeneity of the Neurovascular Unit.Frontiers in cellular neuroscience · 2021Review
- Optical microangiography reveals temporal and depth-resolved hemodynamic change in mouse barrel cortex during whisker stimulation.Journal of biomedical optics · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We describe a method and procedure that allows for the optical coherence tomography angiography (OCTA) and intrinsic optical signal imaging (IOSI) of cerebral blood flow and hemodynamics in fully awake mice. We detail the procedure of chronic cranial window preparation, the use of an air-lift mobile homecage to achieve stable optical recording in the head-restrained awake mouse, and the imaging methods to achieve multiparametric hemodynamic measurements. The results show that by using a collection of OCTA algorithms, the high-resolution cerebral vasculature can be reliably mapped at a fully awake state, including flow velocity measurements in penetrating arterioles and capillary bed. Lastly, we demonstrate how the awake imaging paradigm is used to study cortical hemodynamics in the mouse barrel cortex during whisker stimulation. The method presented here will facilitate optical recording in the awake, active mice and open the door to many projects that can bridge the hemodynamics in neurovascular units to naturalistic behavior.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.