ArticleBiomedical optics express2020
Two-photon image-scanning microscopy with SPAD array and blind image reconstruction.
Article in Biomedical optics express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Breaking the 100-nm resolution barrier with multiphoton microscopy using image scanning microscopy and optical fluctuation imaging.Journal of biomedical optics · 2026Article
- Dual deconvolution in multiphoton structured illumination microscopy for deep-tissue super-resolution imaging.Nature communications · 2026Article
- Wavefront estimation through structured detection in laser scanning microscopy.Biomedical optics express · 2025Article
- Spatiotemporal Exciton Tracking with a SPAD Camera.ACS photonics · 2025Article
- Cell Division Imaging of Tobacco BY-2 Cells in a 3D Volume by Two-Photon Spinning-Disk Confocal Microscopy.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Review
- Deep Mouse Brain Two-Photon Near-Infrared Fluorescence Imaging Using a Superconducting Nanowire Single-Photon Detector Array.ACS photonics · 2024Article
- 4D Single-particle tracking with asynchronous read-out single-photon avalanche diode array detector.Nature communications · 2024Article
- More than double the fun with two-photon excitation microscopy.Communications biology · 2024Review
- Single-photon microscopy to study biomolecular condensates.Nature communications · 2023Article
- Scanning single molecule localization microscopy (scanSMLM) for super-resolution volume imaging.Communications biology · 2023Article
- Temporally resolved SMLM (with large PAR shift) enabled visualization of dynamic HA cluster formation and migration in a live cell.Scientific reports · 2023Article
- Focus image scanning microscopy for sharp and gentle super-resolved microscopy.Nature communications · 2022Article
- The BrightEyes-TTM as an open-source time-tagging module for democratising single-photon microscopy.Nature communications · 2022Article
- Review
- Multiphoton microscopy for label-free multicolor imaging of peripheral nerve.Journal of biomedical optics · 2022Article
- Cooled SPAD array detector for low light-dose fluorescence laser scanning microscopy.Biophysical reports · 2021Article
- Noise and Breakdown Characterization of SPAD Detectors with Time-Gated Photon-Counting Operation.Sensors (Basel, Switzerland) · 2021Article
- Confocal-based fluorescence fluctuation spectroscopy with a SPAD array detector.Light, science & applications · 2021Article
- Article
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two-photon excitation (2PE) laser scanning microscopy is the imaging modality of choice when one desires to work with thick biological samples. However, its spatial resolution is poor, below confocal laser scanning microscopy. Here, we propose a straightforward implementation of 2PE image scanning microscopy (2PE-ISM) that, by leveraging our recently introduced single-photon avalanche diode (SPAD) array detector and a novel blind image reconstruction method, is shown to enhance the effective resolution, as well as the overall image quality of 2PE microscopy. With our adaptive pixel reassignment procedure ∼1.6 times resolution increase is maintained deep into thick semi-transparent samples. The integration of Fourier ring correlation based semi-blind deconvolution is shown to further enhance the effective resolution by a factor of ∼2 - and automatic background correction is shown to boost the image quality especially in noisy images. Most importantly, our 2PE-ISM implementation requires no calibration measurements or other input from the user, which is an important aspect in terms of day-to-day usability of the technique.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.