Evidence map›Paper›PMID 32636482›Full record

ArticleScientific reports2020

Humanized GPRC6A

Min Pi, Fuyi Xu, Ruisong Ye, Satoru K Nishimoto, Robert A Kesterson, Robert W Williams, Lu Lu, L Darryl Quarles

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 16 citations in OpenAlex.

  1. Osteocalcin: A Multifaceted Bone-Derived Hormone.Annual review of nutrition · 2023
    Review
  2. Review
  3. Article
  4. Diverse role of androgen action in human breast cancer.Endocrine oncology (Bristol, England) · 2022
    Review
  5. Review
  6. Review
  7. Review
  8. Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Min PiDepartment of Medicine, University of Tennessee Health Science Center, 19 S Manassas St., Memphis, TN, 38163, USA. mpi@uthsc.edu.
Fuyi XuDepartment of Genetics, Genomics and Informatics, University of Tennessee Health Science Center, 19 S Manassas St., Memphis, TN, 38163, USA.
Ruisong YeDepartment of Medicine, University of Tennessee Health Science Center, 19 S Manassas St., Memphis, TN, 38163, USA.
Satoru K NishimotoDepartment of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, 19 S Manassas St., Memphis, TN, 38163, USA.
Robert A KestersonDepartment of Genetics, University of Alabama at Birmingham, 720 20th Street South, Birmingham, AL, 35294, USA.
Robert W WilliamsDepartment of Genetics, Genomics and Informatics, University of Tennessee Health Science Center, 19 S Manassas St., Memphis, TN, 38163, USA.
Lu LuDepartment of Genetics, Genomics and Informatics, University of Tennessee Health Science Center, 19 S Manassas St., Memphis, TN, 38163, USA.
L Darryl QuarlesDepartment of Medicine, University of Tennessee Health Science Center, 19 S Manassas St., Memphis, TN, 38163, USA. dquarles@uthsc.edu.
University of Tennessee Health Science Center · USUniversity of Alabama at Birmingham · US

Funding

XRAY CRYSTALLOGRAPHYP30CA013148 · NCI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI Robert S Welner · 1985 to 2026
$165.9M
UAB Recessive PKD Research and Translational Core CenterP30DK074038 · NIDDK · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI YODER, BRADLEY K. · 2005 to 2019
$16.6M
ROS Modulation of Innate and Adaptive Immunity in RAP30AR048311 · NIAMS · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI MOUNTZ, JOHN D · 2001 to 2017
$9.5M
UAB Diabetes Research and Training CenterP60DK079626 · NIDDK · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI GARVEY, W TIMOTHY · 2008 to 2012
$8.4M
PHARMACOLOGICALLY INDUCED NEO-OSTEOGENESISR01AR037308 · NIAMS · UNIVERSITY OF TENNESSEE HEALTH SCI CTR · PI QUARLES, L DARRYL · 1991 to 2009
$3.3M
Genetic and Environmental Determinants of GPRC6A Regulation of Energy Metabolism Using Genetically Engineered Mice and Systems BiologyR01DK120567 · NIDDK · UNIVERSITY OF TENNESSEE HEALTH SCI CTR · PI LU, LU, PI, MIN · 2020 to 2023
$2.0M
NCI NIH HHS P30 CA013148NIAMS NIH HHS P30 AR048311NIAMS NIH HHS R01 AR037308NIDDK NIH HHS P30 DK074038NIDDK NIH HHS P60 DK079626NIDDK NIH HHS R01 DK120567NIH HHS P30CA13148NIH HHS R01 DK120567
6 · The paper itself

Abstract

GPRC6A is proposed to regulate energy metabolism in mice, but in humans a KGKY polymorphism in the third intracellular loop (ICL3) is proposed to result in intracellular retention and loss-of-function. To test physiological importance of this human polymorphism in vivo, we performed targeted genomic humanization of mice by using CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9) system to replace the RKLP sequence in the ICL3 of the GPRC6A mouse gene with the uniquely human KGKY sequence to create Gprc6a-

Indexed as

AnimalsBlood GlucoseCRISPR-Associated Protein 9CRISPR-Cas SystemsEnergy MetabolismFibroblast Growth FactorsGene EditingGene Knock-In TechniquesGlucose Tolerance TestHumansInsulinMaleMiceMice, TransgenicPolymorphism, GeneticReal-Time Polymerase Chain ReactionBlood GlucoseCRISPR-Associated Protein 9fibroblast growth factor 21Fibroblast Growth FactorsGPRC6A protein, humanGPRC6A protein, mouseInsulinReceptors, G-Protein-Coupled

Identifiers

PMID32636482
PMCPMC7341878
OpenAlexW3039432972

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.