ArticleScientific reports2020
Sensitive and rapid lateral-flow assay for early detection of subclinical mammary infection in dairy cows.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 25 citations in OpenAlex.
- Prevalence of subclinical mastitis and associated biosecurity practices in peri-urban dairy farms.Open veterinary journal · 2026Article
- Single-step genome-wide association study of milk somatic cell scores across multi-cattle breeds in Ethiopia.Animal biotechnology · 2025Article
- A Rapid and Inexpensive PCR Test for Mastitis Diagnosis Based on NGS Data.Pathogens (Basel, Switzerland) · 2024Article
- Quantum Dot-Based Lateral Flow Immunoassay as Point-of-Care Testing for Infectious Diseases: A Narrative Review of Its Principle and Performance.Diagnostics (Basel, Switzerland) · 2022Review
- Optimizing Effective Parameters to Enhance the Sensitivity of Vertical Flow Assay for Detection ofBiosensors · 2022Article
- Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives.Sensors (Basel, Switzerland) · 2021Review
- A Comparative Study on Changes in Total and Differential Milk Cell Counts, Activity, and Expression of Milk Phagocytes of Healthy and Mastitic Indigenous Sahiwal Cows.Frontiers in veterinary science · 2021Article
- Interaction between stress hormones and phagocytic cells and its effect on the health status of dairy cows: A review.Veterinary world · 2020Review
- Inkjet Printing-Based Immobilization Method for a Single-Step and Homogeneous Competitive Immunoassay in Microchannel Arrays.Frontiers in chemistry · 2020Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Detection of subclinical mastitis (SCM) in its initial stage can save great economic losses, improve milk quality and animal welfare. We have developed a semiquantitative lateral flow assay for the detection of SCM in dairy cows targeting myeloperoxidase (MPO) enzyme of milk neutrophils. A competitive immunoassay format was used, and colloidal gold nanoparticles (GNP) were prepared and used as a labelling agent. Monoclonal anti-MPO antibodies were used and assessed for its quality by enzyme-linked immunosorbent assay and dot blot. Conjugation method for GNP and anti-MPO antibodies was standardised, and the conjugate was placed over the conjugate pad. MPO coupled with a carrier protein (OVA) and the species-specific secondary antibodies were placed on test and control lines, respectively. The developed assay was verified with 75 milk samples collected from healthy, SCM and clinical mastitis cows. It displayed a high sensitivity as it could detect MPO as low as 1.5 ng/ml, an accuracy greater than 97% and showed no crossreactivity when crosschecked with other milk proteins. The developed assay can be used as an alternative for SCM diagnostic tests where lab structure are available for obtaining the lysate of milk SCC.
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