ArticleScientific reports2020
Comparison of methods and characterization of small RNAs from plasma extracellular vesicles of HIV/HCV coinfected patients.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Unveiling the potential of extracellular and outer membrane vesicles in fish aquaculture: a comprehensive review.Frontiers in immunology · 2026Review
- Small extracellular vesicle-associated surface protein biomarkers: emerging roles, opportunities, and challenges in diagnostics.Frontiers in bioengineering and biotechnology · 2025Review
- Mucosal Exosome Proteomics of Hybrid GrouperAnimals : an open access journal from MDPI · 2024Article
- piRNA associates with immune diseases.Cell communication and signaling : CCS · 2024Review
- Identification of RNA-based cell-type markers for stem-cell manufacturing systems with a statistical scoring function.Gene reports · 2024Article
- The extracellular vesicles in HIV infection and progression: mechanisms, and theranostic implications.Frontiers in bioengineering and biotechnology · 2024Review
- MicroRNA signature from extracellular vesicles of HCV/HIV co-infected individuals differs from HCV mono-infected.Journal of molecular medicine (Berlin, Germany) · 2023Article
- Analysis of miRNAs inPharmaceuticals (Basel, Switzerland) · 2023Article
- Methodology for Isolation of miRNA From the Serum of Women Investigated for Pre-eclampsia.Cureus · 2023Article
- Comparison of Extracellular Vesicle Isolation Methods for miRNA Sequencing.International journal of molecular sciences · 2023Article
- Adapter dimer contamination in sRNA-sequencing datasets predicts sequencing failure and batch effects and hampers extracellular vesicle-sRNA analysis.Journal of extracellular biology · 2023Article
- Identifying MicroRNA Markers That Predict COVID-19 Severity Using Machine Learning Methods.Life (Basel, Switzerland) · 2022Article
- MicroRNA let-7 and viral infections: focus on mechanisms of action.Cellular & molecular biology letters · 2022Review
- The involvement of microRNAs in HCV and HIV infection.Therapeutic advances in vaccines and immunotherapy · 2022Review
- Extracellular vesicles isolated by size-exclusion chromatography present suitability for RNomics analysis in plasma.Journal of translational medicine · 2021Article
- Extracellular Vesicles in Immune System Regulation and Type 1 Diabetes: Cell-to-Cell Communication Mediators, Disease Biomarkers, and Promising Therapeutic Tools.Frontiers in immunology · 2021Review
- Elucidating the role of extracellular vesicles in liver injury induced by HIV.Expert review of gastroenterology & hepatologyReview
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hepatitis C virus (HCV) and human immunodeficiency virus (HIV) hijack the host exosomal machinery as an additional mechanism of infection and evasion of the immune system, modifying the small RNA (smRNA) cargo during infection. We characterized the surface epitopes of extracellular vesicles (EVs) from plasma HIV/HCV-coinfected patients and their smRNA cargo profile, by comparing different isolation procedures. Six EVs isolation procedures were compared: ultracentrifugation, and five different polyethylene glycol-based methods (commercial, combined with a column purification step and two custom); and two RNA commercial kits (phenol and non-phenol based) were used. High-throughput sequencing of smRNAs was performed. Exosomal surface epitopes were analyzed by the MACSPlex Exosome Kit. Four miRNAs displayed differences among protocols (hsa-miR-205-5p and hsa-let-7a/b/f-5p). The selection of RNA isolation kit impacted on the detection of miRNAs and other smRNAs, where the phenol-based RNA isolation kit performed acceptably. EVs surface was enriched with HLA-DR/DP/DQ, CD81, and CD8. There were three liver-specific miRNAs overexpressed (let-7a-5p, miR-21-5p and hsa-miR-122-5p), thus, EVs cargo might reflect liver disease evolution. Other smRNAs such as piwi-interacting RNAs were also detected for the first time. Custom polyethylene glycol precipitation-based methods combined with an RNA phenol-based kit yielded the higher number of smRNAs for EVs isolated from plasma HIV/HCV patients.
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