ArticleNature communications2020
Nampt-mediated spindle sizing secures a post-anaphase increase in spindle speed required for extreme asymmetry.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Cumulus cells enhance oocyte genomic quality control by promoting DNA damage-induced meiotic arrest.Journal of cell science · 2026Article
- Intermediate Filament Protein BFSP1 Maintains Oocyte Asymmetric Division by Modulating Spindle Length.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- NAMPT regulates mitochondria and oxidative stress level for mouse early embryo development.Biological research · 2025Article
- The Morphogenesis of Sperm Storage Micropockets in the Western Mosquitofish (Animals : an open access journal from MDPI · 2025Article
- Using FRET to Define Cdk1-Dependent Ordering of Events During Exit from Second Meiotic M-Phase in Oocytes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Article
- NADAging and disease · 2024Article
- Reproductive Ageing: Metabolic contribution to age-related chromosome missegregation in mammalian oocytes.Reproduction (Cambridge, England) · 2024Review
- Can Nicotinamide Adenine Dinucleotide (NADAnimals : an open access journal from MDPI · 2024Review
- Deletion of enzymes for de novo NADAging cell · 2023Article
- NADThe Journal of reproduction and development · 2022Review
- Two-step nuclear centring by competing microtubule- and actin-based mechanisms in 2-cell mouse embryos.EMBO reports · 2022Article
- Insights into the NADThe Journal of reproduction and development · 2022Article
- NAMPT reduction-induced NADAging cell · 2021Article
- Isolation andBio-protocol · 2021Article
- Senataxin: A New Guardian of the Female Germline Important for Delaying Ovarian Aging.Frontiers in genetics · 2021Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Meiotic divisions in oocytes are extremely asymmetric and require pre- and post-anaphase-onset phases of spindle migration. The latter induces membrane protrusion that is moulded around the spindle thereby reducing cytoplasmic loss. Here, we find that depleting the NAD biosynthetic enzyme, nicotinamide phosphoribosyl-transferase (Nampt), in mouse oocytes results in markedly longer spindles and compromises asymmetry. By analysing spindle speed in live oocytes, we identify a striking and transient acceleration after anaphase-onset that is severely blunted following Nampt-depletion. Slow-moving midzones of elongated spindles induce cortical furrowing deep within the oocyte before protrusions can form, altogether resulting in larger oocyte fragments being cleaved off. Additionally, we find that Nampt-depletion lowers NAD and ATP levels and that reducing NAD using small molecule Nampt inhibitors also compromises asymmetry. These data show that rapid midzone displacement is critical for extreme asymmetry by delaying furrowing to enable protrusions to form and link metabolic status to asymmetric division.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.