ArticlemSystems2020
Comparison of Oral Microbiota Collected Using Multiple Methods and Recommendations for New Epidemiologic Studies.
Article in mSystems, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed.
- Long-term stability at -80°C of oral wash and saliva samples for microbiome analyses.Microbiology spectrum · 2026Article
- Site-specific oral microbiome profiles in healthy young Korean women.Scientific reports · 2026Article
- The Mechanism and Pathways of Formation and Modification of Salivary Metabolic Profile in Cancer.Current issues in molecular biology · 2026Review
- Characteristics of Respiratory Microbiome in COPD-A Literature Review.Advances in respiratory medicine · 2026Review
- [The Role of Salivary Microbiota in Oral and Systemic Disease Development and Diagnosis].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2026Review
- Screening for Barrett's esophagus and esophageal adenocarcinoma: recent advances and next steps.Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus · 2026Review
- Standardizing oral microbiome sampling for qPCR: methodological and exploratory insights into nutritional status.Scientific reports · 2026Article
- Association of PCR-based oral bacterial profiles with fluorescence-detected plaque and caries experience in 3-year-old children: a cross-sectional study.BMC oral health · 2026Article
- Comparison of oral collection methods for 16S rRNA gene and shotgun metagenomic sequencing.Microbiology spectrum · 2026Article
- Characterization of the oral microbiome in the diverse South African population by PacBio HiFi sequencing.Applied microbiology and biotechnology · 2026Article
- Oral Microbiome in Oral Cancer Research from Sampling to Analysis: Strategies, Challenges, and Recommendations.Cancers · 2025Review
- Oral Microbiome and Edentulism During Pregnancy: 16S rRNA Gene Analysis of an Indigenous Community-A Pilot Study.Microorganisms · 2025Article
- Stability of oral and fecal microbiome at room temperature: impact on diversity.Frontiers in microbiomes · 2025Article
- Sampling strategies for digestive system flora studies: current research and perspectives.PeerJ · 2025Review
- Is Short-Read 16S rRNA Sequencing of Oral Microbiome Sampling a Suitable Diagnostic Tool for Head and Neck Cancer?Pathogens (Basel, Switzerland) · 2024Article
- Saliva sampling method influences oral microbiome composition and taxa distribution associated with oral diseases.PloS one · 2024Article
- Higher abundance of Campylobacter in the oral microbiome of Japanese patients with moyamoya disease.Scientific reports · 2023Article
- Evaluation of an Adapted Semi-Automated DNA Extraction for Human Salivary Shotgun Metagenomics.Biomolecules · 2023Article
- Stability of the Fecal and Oral Microbiome over 2 Years at -80°C for Multiple Collection Methods.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2023Article
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Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Epidemiologic studies use various biosample collection methods to study associations between human oral microbiota and health outcomes. However, the agreement between the different methods is unclear. We compared a commercially available OMNIgene ORAL kit to three alternative collection methods: Saccomanno's fixative, Scope mouthwash, and nonethanol mouthwash. Oral samples were collected from 40 individuals over 4 visits. Two samples were collected from each subject per visit: one with OMNIgene and one with an alternative method. DNA was extracted using the DSP DNA Virus Pathogen kit, and the V4 region of the 16S rRNA gene was PCR amplified and sequenced using MiSeq. Oral collection methods were compared based on alpha and beta diversity metrics and phylum- and genus-level relative abundances. All alpha diversity metrics were significantly lower for Saccomanno's fixative than for OMNIgene (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.