ArticleBMC microbiology2020
Identifying and exploring biohydrogenating rumen bacteria with emphasis on pathways including trans-10 intermediates.
Article in BMC microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- A Review of Rumen Biohydrogenation and Efficient, Green Production of High-Quality Ruminant Products: Mechanisms, Opportunities and Challenges.Animals : an open access journal from MDPI · 2026Review
- Phenotyping fatty acids and theVeterinary and animal science · 2026Article
- Enhancing the fiber degradation efficiency in dairy cattle rumen through engineered bacterial communities.Microbiome · 2026Article
- Rumen bacteria, carcass traits, and meat quality of growing goat fed diets with different levels of Azolla Pinnata.AMB Express · 2026Article
- Ruminal biohydrogenation and microbial lipids in dairy cows supplemented with fractionated palm oil or Hermetia illucens oil.Journal of animal science · 2026Article
- Multi-omics insights into triticale silage as a sustainable alternative to corn silage in heifer diets.Frontiers in microbiology · 2026Article
- Rumen metabolic environment of dairy cows varying in susceptibility of subacute ruminal acidosis modifies in vitro bacterial composition and fermentation.Scientific reports · 2025Article
- Comparison of the physicochemical properties of palm-based shortenings of various melting temperatures and animal fats.Food chemistry: X · 2025Article
- Conjugated linoleic acid metabolite impact in colorectal cancer: a potential microbiome-based precision nutrition approach.Nutrition reviews · 2025Review
- Isolation ofJDS communications · 2025Article
- Milk Odd- and Branched-Chain Fatty Acids as Biomarkers of Rumen Fermentation.Animals : an open access journal from MDPI · 2024Review
- Progress of Conjugated Linoleic Acid on Milk Fat Metabolism in Ruminants and Humans.Animals : an open access journal from MDPI · 2023Review
- Susceptibility of dairy cows to subacute ruminal acidosis is reflected in both prepartum and postpartum bacteria as well as odd- and branched-chain fatty acids in feces.Journal of animal science and biotechnology · 2022Article
- Unraveling the pros and cons of various in vitro methodologies for ruminant nutrition: a review.Translational animal science · 2022Review
- Integrating data from spontaneous and inducedFrontiers in microbiology · 2022Article
- Assessing the Optimum Level of Supplementation with Camelina Seeds in Ewes' Diets to Improve Milk Quality.Foods (Basel, Switzerland) · 2021Article
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4 authors.
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Abstract
backgroundBacteria involved in ruminal formation of trans-10 intermediates are unclear. Therefore, this study aimed at identifying rumen bacteria that produce trans-10 intermediates from 18-carbon unsaturated fatty acids.
resultsPure cultures of 28 rumen bacterial species were incubated individually in the presence of 40 μg/mL 18:3n-3, 18:2n-6 or trans-11 18:1 under control or lactate-enriched (200 mM Na lactate) conditions for 24 h. Of the 28 strains, Cutibacterium acnes (formerly Propionibacterium acnes) was the only bacterium found to produce trans-10 intermediates from 18:3n-3 and 18:2n-6, irrespective of the growth condition. To further assess the potential importance of this species in the trans-11 to trans-10 shift, different biomass ratios of Butyrivibrio fibrisolvens (as a trans-11 producer) and C. acnes were incubated in different growth media (control, low pH and 22:6n-3 enriched media) containing 40 μg/mL 18:2n-6. Under control conditions, a trans-10 shift, defined in the current study as trans-10/trans-11 ≥ 0.9, occurred when the biomass of C. acnes represented between 90 and 98% of the inoculum. A low pH or addition of 22:6n-3 inhibited cis-9, trans-11 CLA and trans-10, cis-12 CLA formation by B. fibrisolvens and C. acnes, respectively, whereby C. acnes seemed to be more tolerant. This resulted in a decreased biomass of C. acnes required at inoculation to induce a trans-10 shift to 50% (low pH) and 90% (22:6n-3 addition).
conclusionsAmong the bacterial species studied,C. acnes was the only bacterium that have the metabolic ability to produce trans-10 intermediates from 18:3n-3 and 18:2n-6. Nevertheless, this experiment revealed that it is unlikely that C. acnes is the only or predominant species involved in the trans-11 to trans-10 shift in vivo.
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