Evidence map›Paper›PMID 32635856›Full record

ArticleCell cycle (Georgetown, Tex.)2020

Mesenchymal stem cells-derived exosomes ameliorate nucleus pulposus cells apoptosis via delivering miR-142-3p: therapeutic potential for intervertebral disc degenerative diseases.

Lifan Zhu, Yuhui Shi, Ling Liu, Huan Wang, Pengcheng Shen, Huilin Yang

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell cycle (Georgetown, Tex.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
59citing papers in PubMed, 2 pooled it
10.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

59 citing papers in PubMed, 2 syntheses or guidelines pooled it, 87 citations in OpenAlex.

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  14. [Mechanism of extracellular vesicles in the repair of intervertebral disc degeneration].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Lifan ZhuDepartment of Orthopedics, The First Affiliated Hospital of Soochow University , Suzhou, Jiangsu Province, China.
Yuhui ShiDepartment of Orthopedics, The First Affiliated Hospital of Soochow University , Suzhou, Jiangsu Province, China.
Ling LiuDepartment of Orthopedics, The First Affiliated Hospital of Soochow University , Suzhou, Jiangsu Province, China.
Huan WangDepartment of Orthopedics, The First Affiliated Hospital of Soochow University , Suzhou, Jiangsu Province, China.
Pengcheng ShenDepartment of Orthopedics, The First Affiliated Hospital of Soochow University , Suzhou, Jiangsu Province, China.
Huilin YangDepartment of Orthopedics, The First Affiliated Hospital of Soochow University , Suzhou, Jiangsu Province, China.
First Affiliated Hospital of Soochow University · CNSoochow University · CNThe Fifth People’s Hospital of Suzhou · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Intervertebral disc degeneration (IDD) is the main cause of lower back pain (LBP), and puzzles massive individuals worldwide. Mesenchymal stem cells (MSCs) transplantation has been demonstrated to potentially ameliorate IDD progression, while the underlying mechanism has not been fully explained. Interleukin-1β (IL-1β) was used to induce nucleus pulposus cells (NPCs) injury. Bone marrow MSCs-derived exosomes were isolated using the super centrifugation method, and characterized using Transmission electron microscopy (TEM) and western blot. Cell viability was determined by MTT, while apoptosis was measured by Annexin-V staining using flow cytometry. miR-142-3fp and gene expressions were measured by real-time PCR. The protein expressions were determined by western blot. Herein, we found exosomes from bone marrow MSCs are circular vesicles, about 80 nm in diameter, and with robust expression of TSG101 and CD63, but without of Calnexin. MSCs exosomes alleviated NPCs apoptosis by reducing IL-1β-induced inflammatory cytokines secretion and MAPK signaling activation. Additionally, MSCs exosomes inhibited NPCs apoptosis and MAPK signaling by delivering miR-142-3p that targets mixed lineage kinase 3 (MLK3). Overexpression of MLK3 abolished the effects of MSCs exosomes on the inflammatory condition, cell apoptosis, and MAPK signaling activation in NPCs. The results confirmed that bone marrow MSCs-derived exosomes-packaged miR-142-3p alleviates NPCs injury through suppressing MAPK signaling by targeting MLK3. The work highlights the therapeutic effect of MSCs on IDD progression, and bone marrow MSCs exosomes might be apromising therapeutic strategy for IDD.

Indexed as

ApoptosisAnimalsBase SequenceBone Marrow CellsExosomesInflammationInterleukin-1betaIntervertebral Disc DegenerationMAP Kinase Signaling SystemMesenchymal Stem CellsMice, Inbred C57BLMicroRNAsNucleus PulposusInterleukin-1betaMicroRNAsMirn142 microRNA, mouseIntervertebral disc degenerationmiR-142-3pMSCs exosomes

Identifiers

PMID32635856
PMCPMC7469501
OpenAlexW3036668348

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.