ArticleInternational journal of molecular sciences2020
Extracellular Matrix Stiffness and Composition Regulate the Myofibroblast Differentiation of Vaginal Fibroblasts.
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 2 syntheses or guidelines pooled it, 51 citations in OpenAlex.
- Type III Collagen RNA Level Expression in Pelvic Organ Prolapse: A Systematic Review and Meta-Analysis.International urogynecology journal · 2024Pooled it
- The Effect of Growth Factors on Vaginal Wound Healing: A Systematic Review and Meta-analysis.Tissue engineering. Part B, Reviews · 2023Pooled it
- L-Ascorbic Acid 2-Phosphate-Releasing Poly(trimethylene carbonate) Increases Cell Proliferation and Collagen Production: A Novel Material for Pelvic Organ Prolapse Tissue Engineering.Tissue engineering and regenerative medicine · 2026Article
- Mechanobiological Dysregulation of Fibroblasts and Extracellular Matrix Failure in Pelvic Organ Prolapse.International urogynecology journal · 2026Review
- Vaginal aging: from concealed symptoms to defined biomarkers.npj aging · 2026Review
- Assessing Vaginal Wall Elasticity via Shear-Wave Elastography Using a Transrectal Linear Array High-Frequency Probe in Premenopausal versus Postmenopausal Women: A Cross-Sectional Feasibility Study.International journal of women's health · 2026Article
- Unraveling the Causal Linkages ofBioMed research international · 2026Article
- Chapter 4.5: New Proposed Treatments for Pelvic Organ Prolapse.International urogynecology journal · 2026Review
- Decellularization method and tissue region impact the structural properties and composition of porcine vaginal extracellular matrix.Acta biomaterialia · 2026Article
- The Glymphatic-Venous Axis in Brain Clearance Failure: Aquaporin-4 Dysfunction, Biomarker Imaging, and Precision Therapeutic Frontiers.International journal of molecular sciences · 2025Review
- Micro-computed tomography to visualize preserved vascular architecture in decellularized human vaginal tissue: explorative study.Scientific reports · 2025Article
- Multiscale investigation of collagen structure in human skin and gel matrices using polarization resolved second harmonic generation microscopy.Scientific reports · 2025Article
- Role of the integrin‑β1/TGF‑β1 signaling pathway in the pathogenesis of pelvic organ prolapse: A study on vaginal wall tissue alterations and molecular dysfunction.Molecular medicine reports · 2025Article
- Article
- A sterilization method for human decellularized vaginal matrices.Scientific reports · 2024Article
- Exosomes from umbilical cord mesenchymal stromal cells promote the collagen production of fibroblasts from pelvic organ prolapse.World journal of stem cells · 2024Article
- An Improved Understanding of the Pathophysiology of Pelvic Organ Prolapse: A 3D In Vitro Model under Static and Mechanical Loading Conditions.Advanced healthcare materials · 2024Article
- Article
- Effect of human platelet-rich fibrin lysate on collagen type I, collagen type III, and matrix metalloproteinase 1: a protocol study on rat models with pelvic organ prolapse.F1000Research · 2024Article
- Classification of Atretic Small Antral Follicles in the Human Ovary.International journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 4 countries.
Funding
Abstract
Fibroblast to myofibroblast differentiation is a key feature of wound-healing in soft tissues, including the vagina. Vaginal fibroblasts maintain the integrity of the vaginal wall tissues, essential to keep pelvic organs in place and avoid pelvic organ prolapse (POP). The micro-environment of vaginal tissues in POP patients is stiffer and has different extracellular matrix (ECM) composition than healthy vaginal tissues. In this study, we employed a series of matrices with known stiffnesses, as well as vaginal ECMs, in combination with vaginal fibroblasts from POP and healthy tissues to investigate how matrix stiffness and composition regulate myofibroblast differentiation in vaginal fibroblasts. Stiffness was positively correlated to production of α-smooth muscle actin (α-SMA). Vaginal ECMs induced myofibroblast differentiation as both α-SMA and collagen gene expressions were increased. This differentiation was more pronounced in cells seeded on POP-ECMs that were stiffer than those derived from healthy tissues and had higher collagen and elastin protein content. We showed that stiffness and ECM content regulate vaginal myofibroblast differentiation. We provide preliminary evidence that vaginal fibroblasts might recognize POP-ECMs as scar tissues that need to be remodeled. This is fundamentally important for tissue repair, and provides a rational basis for POP disease modelling and therapeutic innovations in vaginal reconstruction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.