ReviewMolecules (Basel, Switzerland)2020
Compensate for or Minimize Matrix Effects? Strategies for Overcoming Matrix Effects in Liquid Chromatography-Mass Spectrometry Technique: A Tutorial Review.
Review in Molecules (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
90 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Calibration Practices in Clinical Mass Spectrometry: Review and Recommendations.Annals of laboratory medicine · 2023Pooled it
- Chemical standardization of kava (Pharmaceutical biology · 2026Article
- Systematic Optimization of an SLE-DAPTAD LC-MS/MS Workflow for Simultaneous Quantification of Low-Abundance Vitamin DRapid communications in mass spectrometry : RCM · 2026Article
- Evaluation and correction of charcoal-stripped serum matrix bias in stable-isotope-dilution LC-MS/MS quantification of endogenous serum folates.Analytical and bioanalytical chemistry · 2026Article
- LC-MS/MS quantification of N-lactoyl-phenylalanine and N-lactoyl-tyrosine in human plasma: method validation and clinical application to metformin administration.Translational and clinical pharmacology · 2026Article
- Health Risk Assessment of Cadmium in Brazilian Makeup Products Using a Novel MDSPE-FAAS Method.ACS omega · 2026Article
- Method development and validation for determination of entinostat in human plasma and its application in breast cancer patients.RSC advances · 2026Article
- Molecularly imprinted polymer-coated metal-organic cages as ambient ionization probes for rapid detection of aflatoxins in peanut and corn.Mikrochimica acta · 2026Article
- Gold nanoclusters-mediated enzyme cascade-amplified immunoassay for dual-mode sensing of aflatoxins.Mikrochimica acta · 2026Article
- Cu-Al-LDH/porphyrin-based COF nanocomposite as a high-performance sorbent for hollow fiber solid phase microextraction of selected pesticide residues.Scientific reports · 2026Article
- ZrOToxins · 2026Article
- Development and validation of matrix-validated LC-MS/MS method for simultaneous quantification of 21 neonicotinoids and their metabolites in human urine.Environmental science and pollution research international · 2026Article
- Article
- Curcuma longa-derived extracellular vesicle-like particles ameliorate retinal neovascularization through HIF-1α and NRF2 signaling.Journal of nanobiotechnology · 2026Article
- Article
- Machine learning prediction and calibration of cellulose-based solid-phase extraction performance for pharmaceuticals across aqueous matrices.RSC advances · 2026Review
- Receptor-Mitochondria Crosstalk in the Kynurenine Metabolic Pathway: Integrating Metabolomics and Clinical Mass Spectrometry.Antioxidants (Basel, Switzerland) · 2026Review
- Analytical Methodologies for Benzo[a]pyrene in Foods: A Review of Advances in Sample Preparation and Detection Techniques.Foods (Basel, Switzerland) · 2026Review
- Targeted LC-MS/MS method for quantifying respiratory pharmaceuticals in wastewater.Environmental science : water research & technology · 2026Article
- Determination of 10 Antibiotics and 53 Plant Growth Regulators in Citrus Fruits by QuEChERS Combined with Liquid Chromatography-Quadrupole/Orbitrap Mass Spectrometry.Foods (Basel, Switzerland) · 2026Article
30 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
In recent decades, mass spectrometry techniques, particularly when combined with separation methods such as high-performance liquid chromatography, have become increasingly important in pharmaceutical, bio-analytical, environmental, and food science applications because they afford high selectivity and sensitivity. However, mass spectrometry has limitations due to the matrix effects (ME), which can be particularly marked in complex mixes, when the analyte co-elutes together with other molecules, altering analysis results quantitatively. This may be detrimental during method validation, negatively affecting reproducibility, linearity, selectivity, accuracy, and sensitivity. Starting from literature and own experience, this review intends to provide a simple guideline for selecting the best operative conditions to overcome matrix effects in LC-MS techniques, to obtain the best result in the shortest time. The proposed methodology can be of benefit in different sectors, such as pharmaceutical, bio-analytical, environmental, and food sciences. Depending on the required sensitivity, analysts may minimize or compensate for ME. When sensitivity is crucial, analysis must try to minimize ME by adjusting MS parameters, chromatographic conditions, or optimizing clean-up. On the contrary, to compensate for ME analysts should have recourse to calibration approaches depending on the availability of blank matrix. When blank matrices are available, calibration can occur through isotope labeled internal standards and matrix matched calibration standards; conversely, when blank matrices are not available, calibration can be performed through isotope labeled internal standards, background subtraction, or surrogate matrices. In any case, an adjusting of MS parameters, chromatographic conditions, or a clean-up are necessary.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.