Evidence map›Paper›PMID 32633898›Full record

ArticleCurrent protocols in cell biology2020

Exosome Isolation by Ultracentrifugation and Precipitation and Techniques for Downstream Analyses.

Christina Coughlan, Kimberley D Bruce, Olivier Burgy, Timothy D Boyd, Cole R Michel, Josselyn E Garcia-Perez, Vanesa Adame, Paige Anton, Brianne M Bettcher, Heidi J Chial and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current protocols in cell biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 174 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
174citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

174 citing papers in PubMed, 1 synthesis or guideline pooled it.

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114 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Christina CoughlanUniversity of Colorado Alzheimer's and Cognition Center, Department of Neurology, Linda Crnic Institute for Down Syndrome, University of Colorado Anschutz Medical Campus, Aurora, Colorado.
Kimberley D BruceDivision of Endocrinology, Metabolism and Diabetes, University of Colorado Anschutz Medical Campus, Aurora, Colorado.
Olivier BurgyDivision of Pulmonary Sciences and Critical Care Medicine, Department of Medicine, University of Colorado Anschutz Medical Campus. INSERM U1231, Faculty of Medicine and Pharmacy, University of Bourgogne-Franche Comté, Dijon, France.
Timothy D BoydUniversity of Colorado Alzheimer's and Cognition Center, Linda Crnic Institute for Down Syndrome, University of Colorado Anschutz Medical Campus, Aurora, Colorado.
Cole R MichelSchool of Pharmacy, University of Colorado Anschutz Medical Campus, Aurora, Colorado.
Josselyn E Garcia-PerezDepartment of Immunology and Microbiology, University of Colorado Anschutz Medical Campus, Aurora, Colorado.
Vanesa AdameUniversity of Colorado Alzheimer's and Cognition Center, Linda Crnic Institute for Down Syndrome, University of Colorado Anschutz Medical Campus, Aurora, Colorado.
Paige AntonDepartment of Pharmaceutical Sciences, University of Colorado Alzheimer's and Cognition Center, Linda Crnic Institute for Down Syndrome, University of Colorado Anschutz Medical Campus, Aurora, Colorado.
Brianne M BettcherUniversity of Colorado Alzheimer's and Cognition Center, Department of Neurology, University of Colorado Anschutz Medical Campus, Aurora, Colorado.
Heidi J ChialUniversity of Colorado Alzheimer's and Cognition Center, Department of Neurology, Linda Crnic Institute for Down Syndrome, University of Colorado Anschutz Medical Campus, Aurora, Colorado.
Melanie KönigshoffDivision of Pulmonary Sciences and Critical Care Medicine, Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, Colorado.
Elena W Y HsiehDepartment of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, Colorado.
Michael GranerDepartment of Pediatrics, Section of Allergy and Immunology, University of Colorado School of Medicine, Children's Hospital Colorado, Aurora, Colorado.
Huntington PotterUniversity of Colorado Alzheimer's and Cognition Center, Department of Neurology, Linda Crnic Institute for Down Syndrome, University of Colorado Anschutz Medical Campus, Aurora, Colorado.

Funding

NIH Prior Approval Process ProfessionalUL1TR002535 · NCATS · UNIVERSITY OF COLORADO DENVER · PI SOKOL, RONALD J. · 2018 to 2022
$51.1M
Institutional Career Development CoreKL2TR002534 · NCATS · UNIVERSITY OF COLORADO DENVER · PI BURNHAM, ELLEN L · 2018 to 2022
$4.6M
Investigating the Contribution of Peripheral versus Central Nervous System Immune Dysfunction to Cognitive AgingR01AG058772 · NIA · UNIVERSITY OF COLORADO DENVER · PI BETTCHER, BRIANNE MAGOUIRK · 2019 to 2023
$3.7M
Immune Dysregulation in Pediatric SLE PathogenesisK23AR070897 · NIAMS · UNIVERSITY OF COLORADO DENVER · PI HSIEH, WEN-YUAN E · 2017 to 2021
$850k
NCATS NIH HHS KL2 TR002534NCATS NIH HHS UL1 TR002535NIAMS NIH HHS K23 AR070897NIA NIH HHS R01 AG058772
6 · The paper itself

Abstract

Exosomes are 50- to 150-nm-diameter extracellular vesicles secreted by all mammalian cells except mature red blood cells and contribute to diverse physiological and pathological functions within the body. Many methods have been used to isolate and analyze exosomes, resulting in inconsistencies across experiments and raising questions about how to compare results obtained using different approaches. Questions have also been raised regarding the purity of the various preparations with regard to the sizes and types of vesicles and to the presence of lipoproteins. Thus, investigators often find it challenging to identify the optimal exosome isolation protocol for their experimental needs. Our laboratories have compared ultracentrifugation and commercial precipitation- and column-based exosome isolation kits for exosome preparation. Here, we present protocols for exosome isolation using two of the most commonly used methods, ultracentrifugation and precipitation, followed by downstream analyses. We use NanoSight nanoparticle tracking analysis and flow cytometry (Cytek

Indexed as

ExosomesProteomicsUltracentrifugationAnimalsChemical PrecipitationNanoparticlesexosomeisolationlipoproteinsmass spectrometryultracentrifugation

Identifiers

PMID32633898
PMCPMC8088761

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.