Evidence map›Paper›PMID 32633191›Full record

ArticlePlant signaling & behavior2020

Generation of Arabidopsis lines with a red fluorescent marker for endoplasmic reticulum using a tail-anchored protein cytochrome

Minoru Nagano, Haruko Ueda, Yoichiro Fukao, Maki Kawai-Yamada, Ikuko Hara-Nishimura

Open access · bronzeAbstract read
In one paragraph

Article in Plant signaling & behavior, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Minoru NaganoCollege of Life Sciences, Ritsumeikan University , Kusatsu, Japan.
Haruko UedaFaculty of Science and Engineering, Konan University , Kobe, Japan.ORCID 0000-0003-3952-076X
Yoichiro FukaoCollege of Life Sciences, Ritsumeikan University , Kusatsu, Japan.
Maki Kawai-YamadaGraduate School of Science and Engineering, Saitama University , Saitama, Japan.
Ikuko Hara-NishimuraFaculty of Science and Engineering, Konan University , Kobe, Japan.ORCID 0000-0001-8814-1593
Konan University · JPRitsumeikan University · JPSaitama University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The endoplasmic reticulum (ER) is a multifunctional organelle that performs multiple cellular activities in eukaryotes. Visualizing ER using fluorescent proteins is a powerful method of analyzing its dynamics and to understand its functions. However, red fluorescent proteins with both an N-terminal signal peptide (SP) and a C-terminal ER retention tetrapeptide (HDEL) often cause mislocalization to vacuoles or extracellular spaces when they are constitutively expressed in Arabidopsis. To obtain a red fluorescent ER marker, we selected Arabidopsis cytochrome

Indexed as

ArabidopsisArabidopsis ProteinsCytochromes b5Endoplasmic ReticulumLuminescent ProteinsRed Fluorescent ProteinArabidopsis ProteinsCytochromes b5Luminescent ProteinsRed Fluorescent Proteincytochrome b5Endoplasmic reticulumred fluorescent proteintail-anchored protein

Identifiers

PMID32633191
PMCPMC8550181
OpenAlexW3040795107

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.