ArticleDoklady. Biochemistry and biophysics2020
Viscous Media Slow Down the Decay of the Key Intermediate in Bacterial Bioluminescent Reaction.
Article in Doklady. Biochemistry and biophysics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 7 citations in OpenAlex.
- The Role of Cosolvent-Water Interactions in Effects of the Media on Functionality of Enzymes: A Case Study ofLife (Basel, Switzerland) · 2023Article
- Structure-Function Relationships in Temperature Effects on Bacterial Luciferases: Nothing Is Perfect.International journal of molecular sciences · 2022Article
- Article
- Mechanisms of Viscous Media Effects on Elementary Steps of Bacterial Bioluminescent Reaction.International journal of molecular sciences · 2021Article
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Authors and funding
4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The effects of medium viscosity on the decay rate of the 4a-hydroperoxyflavin intermediate of the bioluminescent reaction was investigated. It was found that at low concentrations of glycerol or sucrose (viscosity 1.1-1.3 cP) the decay rate rises, whereas a further increase in viscosity to 6.2 cP leads to a decrease in the decay rate following a power function with an exponent of 0.82-0.84. Using molecular dynamics methods, it was shown that the presence of glycerol and sucrose molecules causes a change in the mobility of the amino acid residues in the active center of luciferase, particularly those responsible for binding of flavin. The results obtained are indicative of two opposite effects of viscous media with glycerol and sucrose: (1) destabilization of 4a-hydroperoxyflavin due to a change in the structural and dynamic properties of the protein and (2) stabilization of this intermediate by the decrease in the diffusion rate of its decay products.
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